Optically Sectioned Imaging by Oblique Plane Microscopy

机译：斜面显微镜光学切片成像

获取原文

获取原文并翻译 | 示例

页面导航

摘要
著录项
相似文献
相关主题

摘要

An optically sectioning microscopy technique based on oblique selective plane illumination combined with oblique imaging is described. The same high numerical aperture lens is used to both illuminate and image the specimen and correction optics are employed to tilt the focal plane of the imaging system so that the imaged plane aligns with the illuminated plane in the specimen. An optically sectioned image is obtained without the use of moving parts or image processing and this technique therefore has the potential to be used for very high speed optically sectioned microscopy. As only the part of the specimen that is being imaged is illuminated then the photobleaching and phototoxicity of mis method is low compared to conventional microscopy techniques.

机译：描述了一种基于倾斜选择性平面照明与倾斜成像相结合的光学切片显微镜技术。相同的高数值孔径透镜用于照射和成像样本，并使用校正光学器件倾斜成像系统的焦平面，以使成像平面与样本中的照射平面对齐。无需使用运动部件或进行图像处理即可获得光学切片的图像，因此该技术具有用于超高速光学切片显微镜的潜力。与传统的显微镜技术相比，由于仅照亮了正在成像的标本的一部分，因此光漂白和误涂的光毒性方法较低。

著录项

来源
《Advanced microscopy techniques》|2009年|P.73670H.1-73670H.4|共4页
会议地点 Munich(DE)
作者
C.Dunsby;
展开▼
作者单位

Photonics Group, Department of Physics, Imperial College London, South Kensington Campus, London, SW7 2AZ, UK Department of Histopathology, Division of Investigative Science, Imperial College London, Hammersmith Hospital Campus, London, W12 OHS, UK;

展开▼
会议组织
原文格式 PDF
正文语种 eng
中图分类医用物理学;
关键词
fluorescence microscopy; three-dimensional microscopy; selective plane illumination microscopy;

机译：荧光显微镜三维显微镜选择性平面照明显微镜;

相似文献

外文文献
中文文献
专利

1. Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy [J] . Jiun-Yann Yu, Chun-Hung Kuo, Daniel B. Holland, Journal of biomedical optics . 2011,第11期

机译：平面投影多光子显微镜用于活组织成像的广域光学切片
2. Wide field-of-view volumetric imaging by a mesoscopic scanning oblique plane microscopy with switchable objective lenses [J] . Wenjun Shao, Kivilcim Kilic, Wenqing Yin, Quantitative Imaging in Medicine and Surgery . 2021,第3期

机译：通过具有可切换物镜的介观扫描倾斜平面显微镜宽视野体积成像
3. Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging [J] . Kumar Manish, Kozorovitskiy Yevgenia Optics Letters . 2019,第7期

机译：倾斜不变的扫描斜平面照明显微镜，用于大规模容量成像
4. Optically Sectioned Imaging by Oblique Plane Microscopy [C] . Sunil Kumar, Ziduo Lin, Alex R. Lyon, Three-dimensional and multidimensional microscopy: Image acquisition and processing XVIII . 2011

机译：斜面显微镜光学切片成像
5. Optical Clearing-Assisted Optical Sectioning Microscopy for Point-of-Care Tissue Imaging Applications [D] . Spillane, Derek M. 2020

机译：光学清除辅助光学截面显微镜，用于护理点组织成像应用
6. Wide field-of-view volumetric imaging by a mesoscopic scanning oblique plane microscopy with switchable objective lenses [O] . Wenjun Shao, Kivilcim Kilic, Wenqing Yin, 2021

机译：通过具有可切换物镜的介镜扫描倾斜平面显微镜宽视野体积成像
7. Optically sectioned imaging by oblique plane microscopy [O] . Sunil Kumar, Ziduo Lin, Alex R. Lyon, 2011

机译：倾斜平面显微镜光学切片成像

Optically Sectioned Imaging by Oblique Plane Microscopy

摘要

著录项

相似文献

相关主题

期刊订阅