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首页> 外文会议>Advanced microscopy techniques >Reflective confocal laser scanning microscopy and nonlinear microscopy of cross-linked rabbit cornea
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Reflective confocal laser scanning microscopy and nonlinear microscopy of cross-linked rabbit cornea

机译：交联兔角膜的反射共聚焦激光扫描显微镜和非线性显微镜

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Cross-linking of the cornea with application of Ribovlavin and UV-A light is an evolving clinical treatment of the eye disease keratoconus. Despite the positive clinical track record of corneal cross-linking, the complex wound healing process after the treatment is still under investigation. In this study an animal model was used to clarify the state of wound healing 5 weeks after treatment. Cross-linked rabbit comeae were imaged with reflective confocal laser scanning and nonlinear microscopy, namely second harmonic imaging microscopy (SHIM) and two-photon excited autofluorescence. First results show that the NAD(P)H-autofluorescence of the corneal keratocytes and their scattering signal still show a signature of the treatment five weeks after the cross-linking procedure. The SHIM signals show the structural morphology of the fibrous collagen sheets in the stroma of the cornea. SHIM detected in the forward direction differs substantially from backward SHIM, but no signature of treatment was found in both detection channels of the SHIM signal.
机译：应用核黄素和紫外线-A光使角膜交联是对眼角膜圆锥角膜病的一种不断发展的临床治疗方法。尽管角膜交联的临床记录良好，但治疗后复杂的伤口愈合过程仍在研究中。在这项研究中，使用动物模型来阐明治疗后5周的伤口愈合状态。用反射共聚焦激光扫描和非线性显微镜（即二次谐波成像显微镜（SHIM）和双光子激发自发荧光）对交联的兔彗星进行成像。最初的结果表明，交联程序后五周，角膜角膜细胞的NAD（P）H自发荧光及其散射信号仍然显示出治疗的特征。 SHIM信号显示了角膜基质中纤维状胶原薄片的结构形态。在向前方向检测到的SHIM与向后SHIM基本不同，但是在SHIM信号的两个检测通道中均未发现治疗信号。

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