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Temporal imaging chamber (TIC) for en face imaging of epidermal absorption in vitro

机译：时间成像腔（TIC）用于体外表皮吸收的全脸成像

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Two-photon and confocal microscopy can be used to study the absorption of fluorescent compounds in tissue e.g. for evaluation of topical drug delivery systems. Images of drug distribution at different depth in the skin with relatively high spatial resolution are obtained. However, presented results are often static images from one single time point after topical application.rnWe present an online diffusion cell with optical access allowing for temporal imaging of skin penetration and measurement of percutaneous absorption in vitro. The temporal imaging cell (TIC) is adopted for both two-photon and confocal microscopy. In this study the TIC has been used to visualize the change in absorption of Rhodamine B with time at different depth in human epidermis using two-photon microscopy. Imaging showing the penetration of Rhodamine B with time are presented together with transepidermal fluxes measured in TIC's and in traditional diffusion cells.rnIn conclusion we have shown that the TIC is a promising tool for temporal studies of the absorption of fluorescent compounds in tissue. E.g. sample preparation is held to a minimum prior to imaging, improved detection and resolution in viable epidermis is achieved by imaging from the dorsal side of skin and the possibility to simultaneously analyze the acceptor liquid increases the information available from each experiment.
机译：双光子和共聚焦显微镜可用于研究组织中例如组织中荧光化合物的吸收。用于评估局部给药系统。获得具有较高空间分辨率的在皮肤不同深度的药物分布图像。但是，给出的结果通常是局部应用后一个时间点的静态图像。我们提出了一种具有光学访问功能的在线扩散池，可以对皮肤渗透进行时间成像并在体外测量经皮吸收。双光子显微镜和共聚焦显微镜均采用颞成像细胞（TIC）。在这项研究中，TIC已被用于通过双光子显微镜观察人表皮在不同深度的若丹明B吸收量随时间的变化。图像显示了若丹明B随时间的渗透以及在TIC和传统扩散池中测得的经皮表皮通量。总而言之，我们已经证明TIC是用于组织中荧光化合物吸收的时间研究的有前途的工具。例如。在成像之前将样品准备工作减至最少，通过从皮肤背面进行成像来提高活表皮的检测和分辨率，同时分析受体液体的可能性增加了每个实验可获得的信息。

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