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首页> 外文会议>Advanced microscopy techniques >Barium titanate nanoparticles used as second harmonic radiation imaging probes for cell imaging
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Barium titanate nanoparticles used as second harmonic radiation imaging probes for cell imaging

机译：钛酸钡纳米粒子用作细胞成像的二次谐波辐射成像探针

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摘要

Luminescent markers play a key role in imaging techniques for life science since they provide a contrast mechanism between signal and background. We describe a new type of marker using second harmonic generation (SHG) from noncentrosymmetric BaTiO_3 nanocrystals. These nanoparticles are attractive due to their stable, non-saturating and coherent signal with a femtosecond-scale response time and broad flexibility in the choice of excitation wavelength. In this paper, we report the use of nanoparticles for cell imaging. We first stabilized the BaTiO_3 nanoparticles in suspension and characterized the optical properties. We also demonstrated the functionalization of BaTiO_3 nanoparticles by conjugating IgG antibody on the surface of the nanoparticles. These functionalized nanocrystals are capable of specific labeling the antigens of interest.
机译：发光标记物在生命科学的成像技术中起着关键作用，因为它们提供了信号与背景之间的对比机制。我们描述了一种使用非中心对称BaTiO_3纳米晶体产生二次谐波（SHG）的新型标记。这些纳米粒子具有稳定，非饱和和相干的信号，具有飞秒级的响应时间，并且在选择激发波长时具有广泛的灵活性，因此具有吸引力。在本文中，我们报告了纳米粒子在细胞成像中的应用。我们首先将BaTiO_3纳米颗粒稳定在悬浮液中，并表征了光学性能。我们还通过在纳米颗粒表面缀合IgG抗体证明了BaTiO_3纳米颗粒的功能化。这些功能化的纳米晶体能够特异性标记感兴趣的抗原。

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  • 来源
    《Advanced microscopy techniques》|2009年|P.73670D.1-73670D.6|共6页
  • 会议地点 Munich(DE)
  • 作者

    Chia-Lung Hsieh; Rachel Grange; Ye Pu; Demetri Psaltis;

  • 作者单位

    School of Engineering, EPFL, Station 17,1015 Lausanne, Switzerland Department of Electrical Engineering, California Institute of Technology, 1200 East California Boulevard, MC 136-93, Pasadena, California 91125, USA;

    School of Engineering, EPFL, Station 17,1015 Lausanne, Switzerland;

    School of Engineering, EPFL, Station 17,1015 Lausanne, Switzerland Department of Electrical Engineering, California Institute of Technology, 1200 East California Boulevard, MC 136-93, Pasadena, California 91125, USA;

    School of Engineering, EPFL, Station 17,1015 Lausanne, Switzerland Department of Electrical Engineering, California Institute of Technology, 1200 East California Boulevard, MC 136-93, Pasadena, California 91125, USA;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医用物理学;
  • 关键词

    second harmonic generation; barium titanate; nanoparticles; biological cells; antibody;

    机译：二次谐波产生；钛酸钡纳米粒子生物细胞抗体;

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