首页> 外文会议>Advances in broomcorn millet research : proceedings of the 1st international symposium on broomcorn millet >Factors Affecting Callus Induction on Anther Culture of Proso Millet (Panicum miliaceum L.)
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Factors Affecting Callus Induction on Anther Culture of Proso Millet (Panicum miliaceum L.)

机译:影响Pro谷花药培养愈伤组织诱导的因素

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Proso millet is a promising species for rich nutrition, drought and barren-tolerance. Influences factors on anther-derived callus induction efficiency were investigated when microspores of tested anthers at the late-uninucleate to early-binucleate stage were used as explants. The results indicated the callus induction was strongly affected by genotype. Several highly responsive cultivars of proso millet with desired traits had been identified and they could be directly used in crop improvement by breeding. Cold pre-treatment for three weeks of spikes then 32.5℃ shortly heat post-incubation culture could significant improve callus yield. Moreover, W14 basal medium and combination 60 g/L sucrose with 60 g/L maltose as carbon source were optimal for anther culture of proso millet in this study. To our best knowledge, this is the first report on successful induction of pollen-derived callus in proso millet and it will accelerate the breeding programs.
机译:谷so是一种有前途的物种,具有丰富的营养,干旱和耐旱性。以单核末至双核早期的被试花药小孢子为外植体,研究了影响花药愈伤组织诱导效率的因素。结果表明愈伤组织的诱导受基因型的强烈影响。已经鉴定出具有所需性状的几个早熟谷子高响应品种,它们可以通过育种直接用于作物改良。低温预处理三周穗状花序,然后在培养后不久加热至32.5℃,可以显着提高愈伤组织的产量。此外,在这项研究中,W14基础培养基和60 g / L蔗糖与60 g / L麦芽糖作为碳源的组合是最佳的pro谷花药培养的最佳方法。据我们所知,这是首次在小米中成功诱导花粉衍生的愈伤组织的报告,它将加速育种计划。

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