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Artificial Restriction DNA Cutters (ARCUT) as New Tools to Manipulate Human Genome

机译：人工限制DNA切割机（ARCUT）作为操纵人类基因组的新工具

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We developed artificial restriction DNA cutter (ARCUT) which cuts double-stranded DNA at one desired site. These tools are composed of (i) Ce(IV)/EDTA complex as molecular scissors and (ii) a pair of pseudo-complementary PNAs, and their scission-site is determined by simple Watson-Crick rule. Accordingly, the scission-site and site-specificity are freely chosen by changing the sequences and the lengths of PNA strands. With the use of ARCUT, even huge DNAs (e.g., the whole genome of human beings) are selectively cut at one target site. Furthermore, site-selective scission by ARCUT notably stimulates targeted homologous recombination in human cells.

机译：我们开发了人工限制性DNA切割器（ARCUT），可在一个所需位点切割双链DNA。这些工具由（i）Ce（IV）/ EDTA复合物作为分子剪刀和（ii）一对伪互补PNA组成，其切割位点由简单的Watson-Crick规则确定。因此，通过改变PNA链的序列和长度可以自由选择分裂位点和位点特异性。通过使用ARCUT，甚至可以在一个目标位点选择性切割巨大的DNA（例如，人类的整个基因组）。此外，ARCUT进行的位点选择性切割显着刺激了人类细胞中的靶向同源重组。

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来源
《Advances in biomedical research》|2010年|p.485-489|共5页
会议地点 Cambridge(GB);Cambridge(GB);Cambridge(GB);Cambridge(GB);Cambridge(GB);Cambridge(GB)
作者
JUN SUMAOKA; MAKOTO KOMIYAMA;
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作者单位

Research Center for Advance Science and TechnologyrnThe University of Tokyo 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904rnJAPAN;

Research Center for Advance Science and TechnologyrnThe University of Tokyo 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904rnJAPAN;

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原文格式 PDF
正文语种 eng
中图分类生物医学工程;
关键词
DNA; Cerium(IV); Hydrolysis; Site-selective scission; Artificial Enzyme; PNA; Gene manipulation; Homologous recombination;

机译：脱氧核糖核酸;铈（IV）;水解;选择性选址；人工酶PNA；基因操纵；同源重组;

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专利

1. ARCUT: Artificial Restriction DNA CUTter A powerful tool for gene targeting of human genome [J] . NARUMI SHIGI, MAKOTO KOMIYAMA Chimica oggi: international journal of chemistry and biotechnology . 2010,第1Suppla期

机译：ARCUT：人工限制性DNA切割器一种用于靶向人类基因组的强大工具
2. Artificial Restriction DNA Cutters (ARCUT) for Future Biotechnology [J] . J.Sumaoka, Y.Yamamoto, Y.Kitamura Current organic chemistry . 2007,第5期

机译：未来生物技术的人工限制性DNA切割机（ARCUT）
3. Artificial Restriction DNA Cutters (ARCUT) for Future Biotechnology. [J] . J. Sumaoka Y. Yamamoto Y. Kitamura M. Komiyama Current Organic Chemistry . 2007,第5期

机译：未来生物技术的人工限制性DNA切割剂（ARCUT）。
4. Artificial Restriction DNA Cutters (ARCUT) as New Tools to Manipulate Human Genome [C] . JUN SUMAOKA, MAKOTO KOMIYAMA WSEAS International Conference on Mathematical Biology and Ecology . 2010

机译：人工限制DNA切割器（ARCUT）作为操纵人类基因组的新工具
5. CYTOPLASMIC AND NUCLEAR GENOME DIFFERENTIATION IN A-GENOME DIPLOID SPECIES OF RICE AS REVEALED BY THE RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF DNAS [D] . Ishii, Takashige 1991

机译：DNAs的限制性片段长度多态性分析揭示了水稻A基因组二倍体种的细胞质和核基因组分化
6. Chemical modifications of artificial restriction DNA cutter (ARCUT) to promote its in vivo and in vitro applications [O] . Makoto Komiyama -1

机译：化学修饰人工限制性DNA切割剂（ARCUT）以促进其体内和体外应用
7. An artificial restriction DNA cutter for site-selective gene insertion in human cells [O] . Ito Kenichiro, Shigi Narumi, Komiyama Makoto 2013

机译：用于在人类细胞中进行位点选择基因插入的人工限制性DNA切割器

Artificial Restriction DNA Cutters (ARCUT) as New Tools to Manipulate Human Genome

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