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Biodegradation of C_60 Fullerene Nanowhiskers by Macrophage-like Cells

机译：巨噬细胞样细胞对C_60富勒烯纳米晶须的生物降解作用

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摘要

To evaluate the biological impact of C_(60) fullerene nanowhiskers (C_(60)NWs), an interaction between phorbol 12-myristate 13-acetate (PMA)-treated THP-1 cells (macrophage-like cells) and the C_(60)NWs was investigated in this study. The macrophage-like cells were exposed to 10 ug/mL of C_(60)NWs with an average length of about 6.0 μm and an average diameter of 660 nra. After 1, 3, 6, 12, 24 and 48 h of the exposure, the cells were fixed, stained with Hoechst 33342 and rhodamine-phalloidin and were observed by a differential interference contrast and confocal laser scanning microscope to estimate an uptake rate of C_(60)NWs into cells. To assess the biodegradability of C_(60)NWs by the macrophage-like cells, the cells and the exposed C_(60)NWs were observed by an inverted optical phase-contrast microscope for 28 days after the exposure. After the long-term co-culture of cells and C_(60)NWs, the cells were decomposed by proteinase K and the exposed C_(60)NWs were observed with an optical microscope and a scanning electron microscope to examine the change of C_(60)NWs by the cells. The macrophage-like cells internalized the C_(60)NWs with time and more than 70% of the cells internalized the C_(60)NWs after 48-h exposure. After the long-term co-culture, decomposed C_(60)NWs were observed in the cells and the number of short (less than 3.0 urn in length) C_(60)NWs increased after the exposure. These results suggest that macrophages may be able to decompose C_(60)NWs into C_(60) molecules as the primary immune response.
机译：为了评估C_（60）富勒烯纳米晶须（C_（60）NWs）的生物学影响，佛波醇12-肉豆蔻酸酯13-醋酸酯（PMA）处理的THP-1细胞（巨噬细胞样细胞）与C_（60在本研究中对西北地区进行了调查。将巨噬细胞样细胞暴露于10 ug / mL的C_（60）NW，平均长度约为6.0μm，平均直径为660 nm。暴露1、3、6、12、24和48小时后，将细胞固定，用Hoechst 33342和若丹明-鬼笔环肽染色，并通过微分干涉对比和共聚焦激光扫描显微镜观察，以估计C_的摄取率（60）NW进入细胞。为了评估巨噬细胞样细胞对C_（60）NW的生物降解能力，暴露后28天通过倒置光学相差显微镜观察细胞和暴露的C_（60）NW。细胞和C_（60）NWs长期共培养后，细胞被蛋白酶K分解，并用光学显微镜和扫描电子显微镜观察暴露的C_（60）NWs，以检查C_（60）NWs的变化。 60）牢房的西北。巨噬细胞样细胞随时间内化C_（60）NWs，暴露48小时后超过70％的细胞内化C_（60）NWs。长期共培养后，在细胞中观察到分解的C_（60）NWs，暴露后短（长度小于3.0 um）的C_（60）NWs数量增加。这些结果表明巨噬细胞可能能够将C_（60）NWs分解为C_（60）分子，作为主要的免疫反应。

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  • 来源
    《Advances in biomedical research》|2010年|p.89-94|共6页
  • 会议地点 Cambridge(GB);Cambridge(GB);Cambridge(GB);Cambridge(GB);Cambridge(GB);Cambridge(GB)
  • 作者

    SHIN-ICHI NUDEJIMA; KUNICHI MIYAZAWA; JUNKO OKUDA-SHIMAZAKI; AKIYOSHI TANIGUCHI;

  • 作者单位

    Fullerene Engineering Group, Exploratory Nanotechnology Research LaboratoryrnNational Institute for Materials Science (NIMS)rn1-1 Namiki, Tsukuba, Ibaraki, 305-0044rnJAPAN;

    Fullerene Engineering Group, Exploratory Nanotechnology Research LaboratoryrnNational Institute for Materials Science (NIMS)rn1-1 Namiki, Tsukuba, Ibaraki, 305-0044rnJAPAN;

    Advanced Medical Material Group, Biomaterials CenterrnNational Institute for Materials Science (NIMS)rn1-1 Namiki, Tsukuba, Ibaraki, 305-0044rnJAPAN;

    Advanced Medical Material Group, Biomaterials CenterrnNational Institute for Materials Science (NIMS)rn1-1 Namiki, Tsukuba, Ibaraki, 305-0044rnJAPAN;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物医学工程;
  • 关键词

    Fullerene nanowhisker; Needle-like crystal; Biodegradation; Macrophage; Biological assessment; In vitro;

    机译：富勒烯纳米晶须；针状晶体；生物降解；巨噬细胞；生物评估；体外;

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