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首页> 外文会议>Advances in biomedical research >A Simple Surface Plasmon Resonance Biosensor for Detection and Quantification of Recombinant Human Epidermal Growth Factor (rhEGF) in Escherichia Coli Crude Extract
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A Simple Surface Plasmon Resonance Biosensor for Detection and Quantification of Recombinant Human Epidermal Growth Factor (rhEGF) in Escherichia Coli Crude Extract

机译：一种简单的表面等离子共振生物传感器，用于检测和定量大肠杆菌粗提物中的重组人表皮生长因子（rhEGF）。

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摘要

A reliable detection and quantification assay is important in order to monitor the performance of recombinant human epidermal growth factor (rhEGF) recovery process. Knowing the benefits offered by the surface plasmon resonance (SPR) biosensor, a rhEGF analysis method employing the commercially available BIAcore 3000 equipment was developed by using polyclonal anti-EGF antibody as the capture ligand. The linearity of the assay (determined using the authentic human epidermal growth factor) was found to be in the range of 25 - 250 ng/mL The performance of the developed assay was further evaluated in terms of accuracy, precision (intra and inter-assay), detection and quantification limit. The practical applicability of the assay was justified as a high accuracy (within 10% recovery of the target) and precision (less than 3.4% CV) were obtained for Escherichia coli crude extract samples. The assay was highly reproducible given that the intra- and inter-assay precision obtained were less than 20 % CV. A considerably high sensitivity was also achieved with 8.0 ng/mL of quantification limit.
机译：为了监测重组人表皮生长因子（rhEGF）回收过程的性能，可靠的检测和定量测定很重要。知道表面等离子体共振（SPR）生物传感器提供的好处，通过使用多克隆抗EGF抗体作为捕获配体，开发了使用市售BIAcore 3000设备的rhEGF分析方法。发现测定的线性度（使用真实的人表皮生长因子测定）在25-250 ng / mL范围内。进一步评估了开发测定的性能，涉及准确性，精密度（测定内和测定间） ），检测和定量限。该测定法的实际适用性被证明是正确的，因为它对大肠杆菌粗提物样品具有很高的准确度（在靶标回收率的10％以内）和精密度（低于3.4％CV）。鉴于获得的测定内和测定间精密度小于20％CV，因此该测定具有高度可重复性。 8.0 ng / mL的定量限也获得了相当高的灵敏度。

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