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首页> 外文会议>Advances in chromosome sciences >Detection of alien genes in transgenic rice using fluorescence in situ hybridization
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Detection of alien genes in transgenic rice using fluorescence in situ hybridization

机译：荧光原位杂交技术检测转基因水稻中的外源基因

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摘要

Using multi-color fluorescence in situ hybridization (FISH) , we localized transferred barnase-ps1 and pHctinG DNA sequences onto chromosomes of two transgenic rice plants, named Q12 and Q13, both of which were produced by micro-projectile bombardment. In both Q12 and Q13, each detected cells showed 2 ～ 3 signal spots on their chromosomes, respectively. The signals of both bamase-psl and pHctinG were mostly detected in the adjacent chromosomal sites in which their signals were overlapped and could be recognized by the signal color on the metaphase chromosomes. Fiber FISH further demonstrated that the multiple copies of each of the two DNA sequences distributed adjacently on the DNA fiber in Q13. Combined the results of Southern cmalysts, the possible integration patterns in transgenic rice co-transformed by micro-projectile bombardment had been discussed.
机译：使用多色荧光原位杂交（FISH），我们将barnase-ps1和pHctinG DNA序列定位到两种转基因水稻植株的染色体上，分别命名为Q12和Q13，它们都是通过微弹轰击产生的。在Q12和Q13中，每个检测到的细胞在其染色体上分别显示2〜3个信号点。 bamase-psl和pHctinG的信号大多在相邻的信号重叠的染色体位点检测到，并可以通过中期染色体上的信号颜色识别。 Fiber FISH进一步证明，在Q13中，两个DNA序列中每个序列的多个副本相邻分布在DNA纤维上。结合Southern催化剂的结果，讨论了通过微粒轰击共转化的转基因水稻中可能的整合模式。

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