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首页> 外文会议>Advances in chromosome sciences >Chromosome Studies by Means of Tissue Culture, C-banding Patterns and Physical Mapping in the Genus Allium
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Chromosome Studies by Means of Tissue Culture, C-banding Patterns and Physical Mapping in the Genus Allium

机译：通过组织培养，C带模式和葱属属物理作图的染色体研究

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摘要

We report the combined data for a few decades on the Giemsa C-banding kary-otypes, selection of somaclonal variants by tissue culture, and physical mapping of 5S rRNA gene in the genus Allium. This report is focused on the phylogenetic relationships among Allium species. C-banding patterns by BSG technique are very similar in all species except a few species. Somaclonal variants through tissue culture were obtained in some species and some variants were very useful for breeding. In this situation, the condition of callus induction and regeneration has a considerable difference according to the examined species. Finally, chromosomal localization and distribution patterns of the 5S rRNA gene by means of fluorescence in situ hybridization in the genus Allium can help to classify species into chromosome types that have 5R rRNA gene and aid in determining relationship among genomes.
机译：我们报告了数十年的综合数据，关于吉姆萨（Giemsa）C谱带的核型，通过组织培养选择体细胞克隆变体，以及葱属中5S rRNA基因的物理作图。该报告的重点是葱属物种之间的系统发育关系。除少数物种外，BSG技术的C带模式在所有物种中都非常相似。在某些物种中通过组织培养获得体细胞无性系变体，并且某些变体对于育种非常有用。在这种情况下，根据所检查的物种，愈伤组织的诱导和再生条件存在很大差异。最后，借助于荧光属中的荧光原位杂交技术，5S rRNA基因的染色体定位和分布模式可以帮助将物种分类为具有5R rRNA基因的染色体类型，并有助于确定基因组之间的关系。

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