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Optical thin films serving biotechnology: fluorescence enhancement of DNA-chip

机译：用于生物技术的光学薄膜：DNA芯片的荧光增强

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Over the past years, DNA-chip technology has exploded. Yet scientists using such devices have to face many problems. One of them, due to the very low concentration of biological species to be detected, is the weakness of fluorescence signal collected through the reading system (microscope or scanner). To solve this problem, we proposed to use optical thin films technology. We studied the potentialities of this method step by step. The first step was to be able to understand, explain and forecast the fluorescence emitted by a DNA-chip in terms of fluorescence angular patterns. A theoretical and experimental study enabled us to master this issue even in the case of multi-layers substrates. Using this knowledge we were then able to explain, through simulations, the potentialities of this new type of substrates in terms of fluorescence enhancement. Thus we showed that a theoretical enhancement of twenty-fold (compare to a glass substrate) was achievable.
机译：在过去的几年中，DNA芯片技术迅猛发展。然而使用这种设备的科学家必须面对许多问题。由于要检测的生物物种浓度非常低，其中之一是通过读取系统（显微镜或扫描仪）收集到的荧光信号较弱。为了解决这个问题，我们建议使用光学薄膜技术。我们逐步研究了该方法的潜力。第一步是要能够理解，解释和预测DNA芯片发出的荧光角度图样的荧光。通过理论和实验研究，即使在多层基板的情况下，我们也可以掌握这一问题。然后，利用这些知识，我们可以通过模拟解释这种新型基质在荧光增强方面的潜力。因此，我们表明理论上可以提高20倍（与玻璃基板相比）。

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