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Molecular beacon biosensors for DNA/RNA analysis

机译：用于DNA / RNA分析的分子信标生物传感器

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Abstract: We have developed a variety of novel DNA biosensors using a new class of oligonucleotide probe, molecular beacon (MB). MB has the fluorescence signal transduction mechanism built within the molecules. It can report the presence of specific nucleic acids with high sensitivity and excellent selectivity. Biotinylated MBs have been designed and synthesized for immobilization onto silica surface through avidin-biotin binding. The effect of the avidin-biotin bridge on the MB hybridization has been studied. Our result shows that using streptavidin has less effect than using avidin in MB hybridization. Two kinds of fiber optical DNA sensors have been prepared and characterized: a fiber optic evanescent wave sensor and a submicrometer optical fiber sensor. The sensors are rapid,stable, highly selective, reproducible and regenerable. They have been applied to detect specific DNA and mRNA sequences and to the study of the DNA hybridization kinetics. Silica nanoparticles have also been used for MB immobilization in order to prepare a large quantity of nanometer sized DNA/RNA biosensors.!21
机译：摘要：我们使用一类新型的寡核苷酸探针分子信标（MB）开发了多种新型DNA生物传感器。 MB具有建立在分子内部的荧光信号转导机制。它可以以高灵敏度和出色的选择性报告特定核酸的存在。已经设计并合成了生物素化的MB，以通过抗生物素蛋白-生物素结合固定在二氧化硅表面上。已经研究了抗生物素蛋白-生物素桥对MB杂交的影响。我们的结果表明，在MB杂交中，使用链霉亲和素的效果要比使用亲和素的效果小。已经制备并表征了两种光纤DNA传感器：光纤e逝波传感器和亚微米光纤传感器。传感器快速，稳定，高度选择性，可重现和可再生。它们已被用于检测特定的DNA和mRNA序列以及研究DNA杂交动力学。二氧化硅纳米颗粒也已用于MB固定化，以制备大量的纳米级DNA / RNA生物传感器。21

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