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首页> 外文会议>Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing >Correlation between solution and gas-phase protein conformation: H/D exchange IRMPD and ESI FT-ICR MS
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Correlation between solution and gas-phase protein conformation: H/D exchange IRMPD and ESI FT-ICR MS

机译：溶液和气相蛋白质构象之间的相关性：H / D交换IRMPD和ESI FT-ICR MS

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Abstract: Infrared multiphoton dissociation (IRMPD) of the hydrogen/deuterium (H/D) exchanged 12$PLU charge state of gas-phase bovine ubiquitin was performed on a Fourier transform ion cyclotron resonance mass spectrometer. The H/D exchange of the 12$PLU charge state revealed two distinct isotopic distributions indicating the presence of at least two distinct conformations of the 12$PLU charge state. Following H/D exchange, IRMPD was used to dissociate the conformations. The fragments clearly showed little or no deuterium scrambling as evidenced by a nonstatistical distribution of deuterium incorporation. Analysis of the deuterium incorporation for the five most abundant fragment ions indicated a slow exchanging region of the fast exchanging conformation that corresponds to a stable $beta@- sheet observed by NMR in alcoholic solutions. The data suggest that protection of the amide hydrogens in the $beta@-sheet may result in the observed slow exchange rate and provides further evidence for the retention of secondary structure in gas phase.!39
机译：摘要：在傅立叶变换离子回旋共振质谱仪上进行了气相/牛遍在蛋白氢/氘（H / D）交换的12 $ PLU电荷态的红外多光子离解（IRMPD）。 12 $ PLU电荷状态的H / D交换显示了两个不同的同位素分布，表明存在至少两个不同的12 $ PLU电荷状态的构象。在H / D交换之后，使用IRMPD解离构象。氘掺入的非统计分布清楚地证明了碎片几乎没有氘加扰。对五个最丰富的碎片离子的氘掺入分析表明，快速交换构象的一个缓慢交换区域，相当于在酒精溶液中通过NMR观察到的稳定的β-折叠。数据表明，对$ beta @ -sheet中酰胺氢的保护可能会导致观察到的缓慢交换速率，并为气相保留二级结构提供了进一步的证据！39

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