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首页> 外文会议>Advances in regenerative medicine: Role of nanotechnology. and engineering principles >Chapter 2 Development of Provisional Extracellular Matrix on Biomaterials Interface: Lessons from In Vitro Cell Culture
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Chapter 2 Development of Provisional Extracellular Matrix on Biomaterials Interface: Lessons from In Vitro Cell Culture

机译：第2章在生物材料界面上开发临时细胞外基质：体外细胞培养的经验教训

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摘要

The initial cellular events that take place at the biomaterials interface mimic to a certain extent the natural interaction of cells with the extracellular matrix (ECM). The cells adhering to the adsorbed soluble matrix proteins, such as fibronectin (FN) and fibrinogen (FNG) tend to re-arrange them in fibril-like pattern. Using model surfaces we have demonstrated that this cellular activity is abundantly dependent on the surface properties of materials, such as wettability, surface chemistry, charge and topography. This raises the possibility that tissue compatibility of materials is connected with the allowance of cells to remodel substratum associated proteins presumably to form provisional ECM. We have further shown that antibodies which bind β_1 and α_v integrins (subunits of the FN and FNG receptors respectively) may induce their linear rearrangement on the dorsal surface of living cells - a phenomenon presumably related to the same early molecular events of fibrillar matrix assembly. Because the quantitative measurements revealed that this receptor dynamics is strongly altered on the low compatible (hydrophobic) substrata we hypothesized that in order to be biocompatible, materials need to adsorb matrix proteins loosely, i.e. in such a way that the cells can easily remove and organize them in matrix-like fibrils via coordinated functioning of integrins. More recent studies on the fate of FN on some real biomaterial surfaces, including different rough titanium (Ti) and hydroxyapatite (HA) cements and the surface of biosensors confirmed this point of view. They also show that quantitative measurements of adsorbed matrix proteins and their dynamic rearrangement at cell-material interface might provide insight to the biocompatibility of given material and even predict its tissue integration.
机译：在生物材料界面发生的初始细胞事件在一定程度上模拟了细胞与细胞外基质（ECM）的自然相互作用。粘附于吸附的可溶性基质蛋白（如纤连蛋白（FN）和纤维蛋白原（FNG））的细胞倾向于将它们重新排列为原纤维样模式。使用模型表面，我们证明了这种细胞活性在很大程度上取决于材料的表面特性，例如润湿性，表面化学性质，电荷和形貌。这增加了材料的组织相容性与细胞的允许性相关联以重构基质相关蛋白的可能性，从而可能形成临时的ECM。我们进一步表明，结合β_1和α_v整联蛋白（分别为FN和FNG受体的亚基）的抗体可能会诱导它们在活细胞背侧表面线性重排-这种现象可能与原纤维基质组装的早期分子事件有关。因为定量测量表明该受体动力学在低相容性（疏水性）基质上发生了很大变化，所以我们假设为了具有生物相容性，材料需要松散地吸附基质蛋白，即细胞可以轻松地去除并组织的方式它们通过整联蛋白的协调功能而呈矩阵状原纤维。关于FN在某些实际生物材料表面（包括不同的粗糙钛（Ti）和羟基磷灰石（HA）水泥）以及生物传感器表面的命运的最新研究证实了这一观点。他们还表明，定量测量吸附的基质蛋白及其在细胞-材料界面的动态重排可能为特定材料的生物相容性提供洞察力，甚至可以预测其组织整合。

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  • 来源
  • 会议地点 Varna(BG)
  • 作者

    George Altankov; Thomas Groth; Elisabeth Engel; Jonas Gustavsson; Marta Pegueroles; Conrado Aparicio; Francesc J. Gil; Maria-Pau Ginebra; Josep A. Planell;

  • 作者单位

    Institute of Biophysics Bulgarian Academy of Sciences, Sofia, Bulgaria ICREA (Institucio Catala para Recercia i Estudis Avancats), Barcelona, Spain Institute for Bioengineering of Catalunia, Feixa Llarga Pavello Govern 1°, 1121 08907 L'Hospitalet de Llobregat Barcelona, Spain;

    Department of Pharmacy, Martin-Luther University Halle-Wittenberg, Kurt-Mothes-Strasse 106120, Halle (Saale), Germany;

    Department of Material Science, Universitat Politecnica de Catalunya, Av. Diagonal 64708028,Barcelona, Spain;

    Department of Material Science, Universitat Politecnica de Catalunya, Av. Diagonal 64708028,Barcelona, Spain;

    Department of Material Science, Universitat Politecnica de Catalunya, Av. Diagonal 64708028,Barcelona, Spain;

    Department of Material Science, Universitat Politecnica de Catalunya, Av. Diagonal 64708028,Barcelona, Spain;

    Department of Material Science, Universitat Politecnica de Catalunya, Av. Diagonal 64708028,Barcelona, Spain;

    Department of Material Science, Universitat Politecnica de Catalunya, Av. Diagonal 64708028,Barcelona, Spain;

    Department of Material Science, Universitat Politecnica de Catalunya, Av. Diagonal 64708028,Barcelona, Spain;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物医学工程;
  • 关键词

    biomaterials interface; fibronectin matrix; reorganizattion; provisional ecm; cellular interaction;

    机译：生物材料界面;纤连蛋白基质;重组;临时ecm;细胞相互作用;

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