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PROTEIN-FUNCTIONALIZED PROTON EXCHANGE MEMBRANES

机译:蛋白质功能化质子交换膜

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摘要

Protein-functionalized biomimetic membranes, based upon a triblock copolymer simulating a natural lipid bilayer in a single chain, serves as a core technology for applications in bioenergetics. Monolayers of block copolymer, which simulates the hydrophilic-hydrophobic-hydrophilic chain of a natural cell membrane, can be formed by Langmuir-Blodgett (LB) deposition and provides a favorable environment for protein refolding. Large-scale membrane formation is achieved using LB deposition on a variety of substrates, such as gold, quartz, silicon, and Nafion®. We have successfully inserted membrane proteins, such as the light-activated proton pump, bacteriorhodopsin (BR) and the pH/voltage-gateable porin, Outermembrane Protein F (OmpF), into large-area LB monolayers. We have also established sustained protein functionality in films through the measurement of light-activated proton transport.
机译:基于三嵌段共聚物的单官能模拟天然脂质双层的蛋白质官能化仿生膜,是生物能源学应用的核心技术。可以通过Langmuir-Blodgett(LB)沉积形成模拟天然细胞膜的亲水-疏水-亲水链的嵌段共聚物单层,并为蛋白质重新折叠提供良好的环境。使用LB沉积在各种基材(例如金,石英,硅和Nafion®上)上可实现大规模成膜。我们已经成功地将膜蛋白,例如光激活质子泵,细菌视紫红质(BR)和可通过pH /电压控制的pH值的孔蛋白,外膜蛋白F(OmpF)插入到大面积LB单层中。我们还通过测量光活化质子传输,在胶片中建立了持续的蛋白质功能。

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