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首页> 外文会议>Annual Biochemical Engineering Symposium; 20031005; Ames,IA; US >Production of Bacterial Cellulose by Acetobacter xylinum in Static and Agitated Culture
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Production of Bacterial Cellulose by Acetobacter xylinum in Static and Agitated Culture

机译：木醋杆菌在静态和搅拌培养中生产细菌纤维素

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摘要

The production of bacterial cellulose by Acetobacter xylinum subsp. sucrofermentans in a medium containing sucrose, yeast extract, and mineral salts under static and agitated culture was evaluated. The maximum growth of A. xylinum yielded counts of 2.55 x 10~(10) CFU/ml at 46 h of fermentation in static culture and provided counts of 3.34 x 10~(10) CFU/ml at 45 h of fermentation in agitated culture. In static culture, the yield of cellulose produced increased up until 6 days of cultivation to a value of 1.2 g/l (dry basis). The cellulose yield for agitated culture harvested at 2 days of cultivation was 0.6 g/l (dry basis). Cellulose was produced in the form of a pellicle in static culture, while accumulation of small aggregates of bacterial cellulose was observed in agitated culture. The generation time of A. xylinum in static and agitated cultured was 3.3 and 2.1 h, respectively. The pH of the medium decreased as cultivation time increased in both experiments.
机译：木醋杆菌亚种生产细菌纤维素。在包含蔗糖，酵母提取物和无机盐的培养基中，在静态和搅拌培养下对蔗糖发酵菌进行了评估。木霉的最大生长在静态培养中发酵46 h时产生的计数为2.55 x 10〜（10）CFU / ml，在搅拌培养45 h中产生的计数为3.34 x 10〜（10）CFU / ml。 。在静态培养中，直到培养6天，纤维素的产量才增加到1.2 g / l（干基）。培养2天时收获的搅拌培养物的纤维素产量为0.6g / l（干基）。纤维素在静态培养中以防护膜的形式产生，而在搅拌培养中观察到细菌纤维素小聚集体的积累。木霉在静态和搅拌培养中的生成时间分别为3.3 h和2.1 h。在两个实验中，培养基的pH均随着培养时间的增加而降低。

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