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首页> 外文会议>Annual Biochemical Engineering Symposium; 20031005; Ames,IA; US >Predicting the Performance of Adsorbents based on EDTPA-Modified Zirconia Particles
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Predicting the Performance of Adsorbents based on EDTPA-Modified Zirconia Particles

机译：基于EDTPA改性的氧化锆颗粒的吸附剂性能预测

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A matrix developed from EDTPA-modified zirconia beads (r_PEZ) 25-38 μm in diameter was used to separate immunoglobulins (Ig's). Mechanisms controlling the uptake of Ig's by r_PEZ were studied by static protein uptake experiments and profile modeling with a kinetic rate constant model. Ig binding to r_PEZ was more favorable than dissociation. Breakthrough profiles for human immunoglobulin G (HIgG) uptake were modeled with a pore diffusion model. However, this model barely described profiles for a HIgG concentration of 2 mg/ml, with a theoretical number of transfer units value of approximately 2. Pulse injection techniques provided insights to individual mass transfer parameters. HETP of elution profiles were correlated to salt concentration. Resulting data in conjunction with equations outlined in the literature were used to estimate mass transfer parameters. Results indicate that surface diffusion is absent and pore diffusion is the rate-limiting mechanism in the adsorption of HIgG to r_PEZ.
机译：由直径为25-38μm的EDTPA修饰的氧化锆珠（r_PEZ）开发的基质用于分离免疫球蛋白（Ig）。通过静态蛋白质摄取实验和动力学速率常数模型对模型进行了研究，研究了通过r_PEZ控制Ig摄取的机制。 Ig与r_PEZ的结合比解离更有利。用孔扩散模型对人免疫球蛋白G（HIgG）摄取的突破特征进行建模。但是，该模型几乎没有描述Hmg浓度为2 mg / ml时的分布图，理论上的转移单位数约为2。脉冲注射技术为单个传质参数提供了见识。洗脱曲线的HETP与盐浓度相关。所得数据与文献中概述的方程式一起用于估算传质参数。结果表明，在H_g吸附到r_PEZ上没有表面扩散，而孔扩散是限速机制。

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