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首页> 外文会议>Annual Conference of Japanese Society for Medical and Biological Engineering;Annual International Conference of the IEEE Engineering in Medicine and Biology Society >Softening of the mouse zona pellucida during oocyte maturation
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Softening of the mouse zona pellucida during oocyte maturation

机译：卵母细胞成熟过程中小鼠透明带的软化

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摘要

A change in the elasticity and the resistance to dissolution of the mouse zona pellucida (ZP) was quantitatively evaluated at immature germinal vesicle (GV), mature metaphase II (MII) and fertilized pronuclear (PN) stages. Young's modulus of the ZP was measured using a micro tactile sensor (MTS), a highly sensitive resonator-based sensor for a micro scale elasticity measurement. 0.25% α-chymotrypsin was used for the ZP dissolution assay. The results of measuring the ZP elasticity and the dissolution time clearly showed that the ZP softened during oocyte maturation and the ZP hardened after fertilization. The results indicate that the amount of the zona softening can be a criterion to evaluate oocyte quality for the selection of top quality mature oocyte before in vitro fertilization (IVF) treatment.
机译：在未成熟的生小泡（GV），成熟的中期II（MII）和受精的原核（PN）阶段，定量评估了小鼠透明带（ZP）的弹性和抗溶解性的变化。 ZP的杨氏模量是使用微触觉传感器（MTS）进行测量的，该传感器是用于微尺度弹性测量的基于高灵敏度谐振器的传感器。将0.25％α-胰凝乳蛋白酶用于ZP溶解测定。测定ZP弹性和溶解时间的结果清楚地表明，ZP在卵母细胞成熟过程中软化，而ZP在受精后硬化。结果表明，透明带的软化量可以作为评估卵母细胞质量的标准，以在体外受精（IVF）处理之前选择顶级质量的成熟卵母细胞。

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