The multigene dirigent family of sitka spruce (Picea sitchensis): differential gene expression in response to wounding

机译：西特卡云杉（Picea sitchensis）的多基因dirigent家族：响应受伤的差异基因表达

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We have isolated, cloned and characterized the expression of the multigene dirigent family from Sitka Spruce (Picea sitchensis). To-date, this family consists of dirigent genes that can be separated into two distinct clads by phylogenetic analysis. One clad was shown to be spatially and temporally up-regulated in response to external stimuli, including mechanical wounding and insect attack, while the other clad seem to be display significantly higher basal levels of gene expression. The wound-inducible clad possess coding regions of 194-195 amino acids with a predicted molecular mass of ca. 22 - 23 kDa, and all contain a putative hydrophobic signal peptide as part of their secretory system. The primary structure of the isoforms displayed a high degree of amino acid homogeneity (81-91%), while the deduced isoelectric points for the isoforms ranged from 5.4 to 7.9.

机译：我们已经分离，克隆和表征了来自Sitka云杉（Picea sitchensis）的多基因dirigent家族的表达。迄今为止，该家族由不同的基因组成，可以通过系统发育分析将其分成两个不同的包覆层。一个包层显示出对外部刺激（包括机械性伤害和昆虫攻击）的反应在空间和时间上调，而另一个包层似乎显示出更高的基础基因表达水平。伤口诱导包层具有194-195个氨基酸的编码区，预测的分子量约为1。 22-23 kDa，并且都包含一个假定的疏水信号肽作为其分泌系统的一部分。同工型的一级结构显示出高度的氨基酸同质性（81-91％），而推导的同工型等电点范围为5.4至7.9。

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《Appita Annual Conference and Exhibition incorporating the ISWFPC(International Symposium on Wood, Fibre and Pulping Chemistry) vol.2: 13th ISWFPC Sessions - Oral Papers; 20050516-19; Auckland(NZ)》|2005年|P.181-187|共7页
会议地点 Auckland(NZ)
作者
JI-YOUNG PARK; STEVEN RALPH; JOERG BOHLMANN; SHAWN D. MANSIELD;
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Wood and Fibre Quality, Department of Wood Science University of British Columbia, Vancouver, BC, Canada V6T 1Z4;

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正文语种 eng
中图分类造纸工业;
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The multigene dirigent family of sitka spruce (Picea sitchensis): differential gene expression in response to wounding

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