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In-vitro fertilisation: a simple, efficient method for obtaining sea cucumber larvae year round

机译:体外受精:一种简单,有效的全年获取海参幼虫的方法

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摘要

Obtaining eggs and larvae in large quantities is a critical point for the economic viability of sea cucumber aquaculture. In this paper, spawning induction methods and in-vitro fertilisation (IVF) methods are presented and compared. The IVF technique developed in Madagascar (MH-IVF) is a simple, cost-efficient method that enables hatcheries to obtain clean, fertilised eggs of sea cucumbers year-round. MH-IVF does not require high-tech equipment and is applicable in small- and large-scale hatcheries. It ensures the best control at the very beginning of the work on the number and type of genitors (i.e. sex, length, weight, colour); the quality of the gonads (healthy versus parasitised); and the number, size and quality of spermatozoa and eggs. MH-IVF involves the sacrifice of very few genitors compared with the individuals obtained and sacrificed for production. Yet, it does not influence genetic drift any more than spawning induction methods.
机译:大量获取卵和幼虫是海参养殖经济可行性的关键点。本文提出并比较了产卵诱导方法和体外受精(IVF)方法。马达加斯加开发的IVF技术(MH-IVF)是一种简单,经济高效的方法,可使孵化场全年获得干净,受精的海参卵。 MH-IVF不需要高科技设备,并且适用于小型和大型孵化场。它确保在一开始就对生殖器的数量和类型(即性别,身长,体重,肤色)进行最佳控制;性腺的质量(健康与寄生);以及精子和卵的数量,大小和质量。与获得和牺牲生产的个体相比,MH-IVF牺牲了很少的生殖器。但是,它仅对产卵诱导方法产生影响。

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  • 会议地点 Noumea(NC)
  • 作者单位

    Laboratoire de Biologie Marine, Universite de Mons, Mons, Belgique,Madagascar Holothurie S.A., Toliara, Madagascar;

    Madagascar Holothurie S.A., Toliara, Madagascar,Institut Halieutique et des Sciences Marines, Universite de Toliara, Toliara, Madagascar;

    Laboratoire de Biologie Marine, Universite de Mons, Mons, Belgique;

    Laboratoire de Biologie Marine, Universite Libre de Bruxelles, Bruxelles, Belgique;

    Institut Halieutique et des Sciences Marines, Universite de Toliara, Toliara, Madagascar;

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