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首页> 外文会议>Asian-Australasian Conference on Composite Materials;ACCM; 20061127-30;20061127-30; Hong Kong(CN);Hong Kong(CN) >Bio-engineered Mesenchymal Stem Cell-Tricalcium Phosphate Ceramics Composite Augmented Bone Regeneration in Posterior Spinal Fusion
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Bio-engineered Mesenchymal Stem Cell-Tricalcium Phosphate Ceramics Composite Augmented Bone Regeneration in Posterior Spinal Fusion

机译：生物工程间充质干细胞-磷酸三钙陶瓷复合材料在后路脊柱融合中增强骨再生。

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Grafting of autologous iliac crest and decortication approach in posterior spinal fusion surgery has been the "gold standard". However, the limited source of autograft has prompted extensive research into bone substitute and biological enhancement of the fusion mass. In this study, the application of stem cell therapy by tissue engineering method was investigated to enhance posterior spinal fusion with P-tricalcium phosphate ceramics in rabbit model. Rabbit bone marrow derived mesenchymal stem cells were aspirated from trochanter region of proximal femur. The mesenchymal stem cells were grown and directed to differentiate into osteogenic cells by osteogenic supplement (ascorbic acid, P-glycerophosphate and dexamethasone) in basal medium (10% FBS in DMEM). The osteogenic cells were seeded on tricalcium phosphate ceramics for one day (MSC group, n=6). The cell-ceramics composite was implanted onto autologous L5 and L6 transverse processes with decortication approach in posterior spinal fusion. The cell free ceramics acts as control (Control group, n=6) and iliac crest autograft as positive control (Autograft group). The spinal segments were harvested at week 7 post-operation. Manual palpation was performed with spinal segments to assess any movement of L5-L6 vertebral joint. The stiffness of the joint was considered as solid fusion. The specimens then were fixed by formalin and transferred to 70% ethanol. The BMC and volume of fusion transverse processes of L5 and L6 was measured by peripheral quantitative computed tomography. In manual palpation, 50% solid fusion was found in MSC group, 60% in autograft group but none in control group. Moreover, the BMC of L5 and L6 transverse processes in MSC group was greater than autograft and control group (45%, 40% respectively, p<0.01). The volume of transverse processes in MSC group was greater than autograft by 45% (p<0.01) and control group by 26% (p<0.05). In conclusion, the mesenchymal stem cells derived osteogenic cells augmented spinal fusion and bone mineralization.
机译：在后路脊柱融合手术中自体c的嫁接和脱皮方法已成为“金标准”。然而，自体移植的有限来源促使人们对骨替代物和融合质量的生物学增强进行了广泛的研究。在这项研究中，研究了组织工程方法在干细胞治疗中的应用，以增强P-磷酸三钙陶瓷在兔模型中的后路脊柱融合。从股骨近端转子区抽吸兔骨髓来源的间充质干细胞。间充质干细胞在基础培养基（DMEM中为10％FBS）中生长，并通过成骨性补充剂（抗坏血酸，P-甘油磷酸盐和地塞米松）定向分化为成骨细胞。将成骨细胞接种在磷酸三钙陶瓷上一天（MSC组，n = 6）。在后路脊柱融合术中，用去皮法将细胞-陶瓷复合材料植入自体L5和​​L6横向过程。无细胞陶瓷作为对照（对照组，n = 6），auto自体移植作为阳性对照（自体移植组）。术后第7周收获脊髓节段。对脊柱节段进行手动触诊，以评估L5-L6椎骨关节的任何运动。关节的刚度被认为是固体融合。然后将样品用福尔马林固定并转移至70％乙醇中。 LMC和L5融合横向过程的BMC和体积通过外围定量计算机断层扫描测量。在人工触诊中，MSC组中发现了50％的固体融合，自体移植组中发现了60％，而对照组中没有。此外，MSC组L5和L6横突的BMC大于自体移植组和对照组（分别为45％，40％，p <0.01）。 MSC组的横突量比自体移植大45％（p <0.01），而对照组则大于26％（p <0.05）。总之，间充质干细胞衍生的成骨细胞增强了脊柱融合和骨矿化作用。

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