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Video imaging in flow - a novel method for increasing the discriminating power of flow cytometers used in aquatic science

机译:流中的视频成像-一种增加水生科学中流式细胞仪辨别能力的新颖方法

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Abstract: The procedure of imaging in flow, evoked by the need for simplified and reliable particle identification in aquatic science, means taking cell images directly from the transducer of a flow cytometer. From that point of view imaging in flow is the link of the traditional microscopical method and the modern flow cytometric technique for analyzing particles. Principal problems rising from that approach are discussed in this paper. This encloses positioning of the objects to be imaged, design criteria for illumination and imaging optics, the procedure for taking asynchronous video images and requirements concerning the light source. !12
机译:摘要:由于需要简化和可靠的水产科学中的颗粒识别而引起的流动成像过程意味着直接从流式细胞仪的换能器获取细胞图像。从这个角度来看,流动成像是传统的显微镜方法和现代的流式细胞仪技术分析颗粒的联系。本文讨论了这种方法引起的主要问题。这包括要成像对象的定位,照明和成像光学器件的设计标准,获取异步视频图像的过程以及与光源有关的要求。 !12

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