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Selenium volatilization by soil bacteria isolated from rhizosphere of rabbitfoot grass (Polypogon monspeliensis)

机译:兔足草(Polypogon monspeliensis)根际土壤细菌对硒的挥发作用

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Rabbitfoot grass (Polypogon monspeliensis) was previously identified as one of the most effective plant species for selenium (Se) volatilization in treatment wetlands under field conditions. Both plants and soil microbes are capable of methylating inorganic Se into volatile selenide compounds, which varies significantly among the species. Therefore, it is important to identify the soil mi-crobes that have the greatest ability of volatilizing Se in the soil- rabbitfoot grass system. In this laboratory study, a total 18 soil bacterial strains were isolated from top and rhizosphere soils of rabbitfoot grass that were previously treated with 20 mg(Se)· kg<'1>, as well as from those of the control (background Se level <1 mg(Sc)·kg <'1>). The rate of Se volatilization was deter-mined in 200 ml pure bacterial culture solutions containing 6mg(Se)· L<'1>. Results show that Cellulornonas cellasea isolated from the Se-treated soil rabbitfoot grass system produced the highest rate of Sc volatilization (638μg Se per flask per day), followed by Listeria grayi, 112μg Se per flask per day.
机译:兔脚草(Polypogon monspeliensis)先前被确定为田间条件下处理湿地中硒(Se)挥发最有效的植物之一。植物和土壤微生物都能够将无机硒甲基化为挥发性硒化物,这在物种之间差异很大。因此,重要的是确定在土壤-兔脚草系统中具有最大的Se挥发能力的土壤微生物。在该实验室研究中,从兔脚草的顶部和根际土壤中分离出总共18种土壤细菌菌株,这些土壤细菌先前已用20 mg(Se)·kg <1>处理过,以及从对照土壤中(本底硒水平<1 mg(Sc)·kg <'1>)。在200ml含有6mg(Se)·L 1的纯细菌培养液中确定Se的挥发速率。结果表明,从Se处理过的土壤兔脚草系统中分离到的Cellulornonas cellasea产生最高的Sc挥发速率(每瓶每天638μgSe),其次是李斯特菌,每瓶每天112μgSe。

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