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首页> 外文会议>The 2nd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2008)(第二届生物信息与生物医学工程国际会议）论文集 >Preparation of Nano-sized Collagen Ⅰ and Collagen Ⅱ Particles
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Preparation of Nano-sized Collagen Ⅰ and Collagen Ⅱ Particles

机译：纳米Ⅰ型胶原蛋白和Ⅱ型胶原蛋白颗粒的制备

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摘要

This pilot study describes a new method to prepare nano-sized collagen type Ⅰ and collagen type Ⅱ particles by using a high-voltage electrostatic field system. Observations from transmission electron microscopy showed that, the nano-sized collagen II particles with a range of 150 nm in diameter could be produced with a good spherical shape under the experimental setting of 2.7～4.5 kVcm-1, under 23℃ and a moderate concentration of collagen Ⅱ solution at 0.2 mg/ml. When the treatment temperature increased to 30℃ or higher, collagen Ⅱ began to lose the tendency to individually separated spherical shaped nano-sized particles, instead, the fibrillogenesis of collagen Ⅱ occurred by the spontaneous reconstitution of collagen Ⅱ and then formed collagen Ⅱ fibrils. More strict experimental settings were necessary for preparing nano-sized collagen I particles, that are, lower temperature setting (under 7℃ environment), lower collagen concentration (0.1 mg/ml) and lower applied voltage (2.5 kVcm-1). Moreover, these prepared nano-sized collagen I particles exhibited smaller size in diameter (100 nm) but poorer sphericity than collagen Ⅱ particles. They often aggregated together and exhibited an irregular outer shape.
机译：这项初步研究描述了一种通过高压静电场系统制备纳米级Ⅰ型胶原和Ⅱ型胶原颗粒的新方法。透射电镜观察表明，在2.7〜4.5 kVcm-1、23℃，中等浓度的实验条件下，可以制备出良好球形的纳米直径II纳米胶原II颗粒，粒径为150nm。胶原蛋白Ⅱ溶液的浓度为0.2 mg / ml。当处理温度升至30℃或更高时，Ⅱ型胶原开始失去单独分离球形纳米颗粒的趋势，取而代之的是，Ⅱ型胶原的自发重组发生了Ⅱ型胶原的原纤维形成，然后形成了Ⅱ型胶原原纤维。制备纳米胶原I颗粒需要更严格的实验设置，即较低的温度设置（在7℃环境下），较低的胶原蛋白浓度（0.1 mg / ml）和较低的施加电压（2.5 kVcm-1）。此外，这些制备的纳米级胶原蛋白I颗粒的直径（100 nm）较小，但球形度却比胶原蛋白Ⅱ颗粒差。它们经常聚集在一起，并呈现出不规则的外形。

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