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首页> 外文会议>Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on >Comparison of the Major Flavonoid Content of Raw and Steamed Scutellariae Radix by High-Performance Liquid Chromatography
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Comparison of the Major Flavonoid Content of Raw and Steamed Scutellariae Radix by High-Performance Liquid Chromatography

机译：高效液相色谱法比较黄cut和生黄cut的主要黄酮含量

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摘要

This study was conducted to evaluate the effects of different extraction methods on the major flavonoid content of raw and steamed Scutellariae radix by applying high-performance liquid chromatography. Eight extraction methods such as extraction method in 2010 Chinese Pharmacopoeia (ChP-method), water refluxing extraction (WTRE-method), 60% ethanol refluxing extraction (ETRE-method), ultrasonic-assisted aqueous extraction (UAAE-method), ultrasonic-assisted 60% ethanol extraction (UAEE-method), cellulase-assisted solvent extraction (CASE-method), pectinase-assisted solvent extraction (PASE-method), and double-enzyme-assisted solvent extraction (DEASE-method) were investigated. The content ranges of baicalin (BG), wogonoside (WG), baicalein (BA), and wogonin (WO) in raw Scutellariae radix by using ChP-, ETRE-, UAEE-, WTRE-method were 194.91~226.50, 69.90~77.11, 33.30~68.83, and 15.83~20.40 µmol g-1 herbals, respectively, while those by using UAAE-, CASE-, PASE-, DEASE-method were 3.81~7.17, 14.34~26.50, 134.70~227.58, and 42.92~46.79 µmol g-1 herbals, respectively. The result showed that the effects of different extraction methods on the major flavonoid content in raw Scutellariae radix were very significant. However, the content ranges of BG, WG, BA and WO in steamed Scutellariae radix by using eight extraction methods were 196.48~239.94, 67.99~79.93, 33.30~71.42, and 9.85~22.87 µmol g-1 herbals, respectively. Compared with raw Scutellariae radix, the effects of different extraction methods on the major flavonoid content in steamed Scutellariae radix were not significant. Based on these findings, the extraction method and the extraction solvent selected could influence the content of the bioactive flavonoids extracted from Scutellariae radix, and the processing could also do it.
机译：本研究旨在通过应用高效液相色谱法评估不同提取方法对生和蒸黄根中主要黄酮含量的影响。八种提取方法，例如2010年中国药典的提取方法（ChP方法），水回流提取（WTRE方法），60％乙醇回流提取（ETRE方法），超声辅助水提取（UAAE方法），超声提取研究了辅助60％乙醇萃取（UAEE方法），纤维素酶辅助溶剂萃取（CASE方法），果胶酶辅助溶剂萃取（PASE方法）和双酶辅助溶剂萃取（DEASE方法）。通过ChP-，ETRE-，UAEE-，WTRE方法测定黄S中的黄ical苷（BG），皂甙（WG），黄ical素（BA）和沃戈宁（WO）的含量范围为194.91〜226.50，69.90〜77.11 ，33.30〜68.83和15.83〜20.40 µmol g-1草药，而使用UAAE-，CASE-，PASE-，DEASE方法的分别为3.81〜7.17、14.34〜26.50、134.70〜227.58和42.92〜46.79 µmol g-1草药。结果表明，不同提取方法对生黄raw中主要黄酮含量的影响非常显着。然而，八种蒸方法提取的黄S中BG，WG，BA和WO的含量范围分别为196.48〜239.94、67.99〜79.93、33.30〜71.42和9.85〜22.87 µmol g-1。与生黄S相比，不同提取方法对蒸黄S中主要黄酮含量的影响不显着。基于这些发现，提取方法和提取溶剂的选择可能会影响从黄S中提取的生物活性类黄酮的含量，并且加工也可以做到这一点。

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