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Three-dimensional imaging of micro-specimen by optical scanning holography

机译:显微样品的光学扫描全息三维成像

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Optical scanning holography (OSH) is a scanning-type digital holographic technique. In OSH, a heterodyne interference pattern is generated to raster scan the object. OSH can be operated in the incoherent mode and thus is able to record a fluorescence hologram. In addition, resolution of the OSH is proportional to the density of the interference pattern. Here we use a high-NA microscope objective to generate a dynamic Fresnel zone plate to record a hologram of micro-specimen. The achieved transverse resolution and longitudinal resolution are 0.78μm and 3.1μm , respectively.
机译:光学扫描全息(OSH)是一种扫描型数字全息技术。在OSH中,生成外差干涉图样以对对象进行光栅扫描。 OSH可以在非相干模式下运行,因此能够记录荧光全息图。另外,OSH的分辨率与干涉图样的密度成正比。在这里,我们使用高NA显微镜物镜来生成动态菲涅耳波带片,以记录显微样品的全息图。达到的横向分辨率和纵向分辨率分别为0.78μm和3.1μm。

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