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Cell free translation in engineered picoliter volume containers

机译:工程化皮升容量容器中的无细胞翻译

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Engineers seek to use biological design principles to manipulate information and import new functionality to synthetic devices. Such devices inspired by natural systems could, in turn, play a crucial role in allowing biologists to explore the effects of physical transport and extreme conditions of temperature and pH on reaction systems. For example, engineered reaction containers can be physically and chemically defined to control the flux of molecules of different sizes and charge. The design and testing of such a container is described here. It has a volume of 19 pL with defined slits of 200 nm. The device successfully contained DNA and protein molecules and is evaluated for carrying out cell-free protein synthesis. The effect of DNA concentration and slit size on protein yield is discussed.
机译:工程师寻求利用生物学设计原理来操纵信息并将新功能导入合成设备。反过来,受自然系统启发的此类设备在使生物学家探索物理迁移以及温度和pH的极端条件对反应系统的影响方面可能起关键作用。例如,工程反应容器可以在物理和化学上进行定义,以控制不同大小和电荷的分子的通量。这里描述了这种容器的设计和测试。它的体积为19 pL,定义的缝隙为200 nm。该装置成功地包含了DNA和蛋白质分子,并进行了无细胞蛋白质合成的评估。讨论了DNA浓度和狭缝大小对蛋白质产量的影响。

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