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首页> 外文会议>Biomedical vibrational spectroscopy 2016: Advances in research and industry >Development of an Optical Biosensor Based on Surface-Enhanced Raman Scattering for DNA Analysis
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Development of an Optical Biosensor Based on Surface-Enhanced Raman Scattering for DNA Analysis

机译：基于表面增强拉曼散射的DNA分析光学生物传感器的开发

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摘要

Rapid, accurate and sensitive DNA analysis is critically important for the diagnostic of genetic diseases. The most common method preferred in practice is fluorescence based microarrays to analyze the DNA. However, there exist some disadvantages related to the above-mentioned method such as the overlapping of the fluorescence emission wavelengths that can diminish in the performance of multiplexing, needed to obtain fluorescence spectra from each dye and photo degradation. In this study, a novel SERS based DNA analysis approach, which is Raman active dye-free and independent of SERS substrate properties, is developed. First, the single strand DNA probe is attached to the SERS substrate and half of the complimentary DNA is attached to gold nanoparticles, as well. We hypothesize that in the presence of target DNA, the complimentary DNA coupled colloids will bind to the SERS substrate surface via hybridization of single strand target DNA. To test this hypothesis, we used UV/Vis spectroscopy, atomic for microscopy (AFM) and dynamic light scattering (DLS). DNA analysis is demonstrated by a peak shift of the certain peak of the small molecules attached to the SERS substrate surface instead of SERS spectrum obtained in the presence of target DNA from the Raman reporter molecules. The degree of peak shifting will be used for the quantification of the target DNA in the sample. Plasmonic properties of SERS substrates and reproducibility issues will not be considerable due to the use of peak shifting instead of peak intensity for the qualitative analysis.
机译：快速，准确和敏感的DNA分析对于遗传疾病的诊断至关重要。在实践中，最常用的方法是基于荧光的微阵列分析DNA。但是，存在与上述方法有关的一些缺点，例如，荧光发射波长的重叠可能会降低多路复用的性能，需要从每种染料获得荧光光谱并进行光降解。在这项研究中，开发了一种新颖的基于SERS的DNA分析方法，该方法无拉曼活性染料，并且与SERS底物特性无关。首先，将单链DNA探针连接到SERS底物，一半互补DNA也连接到金纳米颗粒。我们假设在目标DNA的存在下，互补的DNA偶联胶体将通过单链目标DNA的杂交与SERS底物表面结合。为了验证这一假设，我们使用了紫外/可见光谱，原子显微镜（AFM）和动态光散射（DLS）。 DNA分析通过附着在SERS底物表面的小分子的某些峰的峰位移而不是在存在来自拉曼报告分子的目标DNA的情况下获得的SERS光谱证明。峰移动的程度将用于定量样品中的目标DNA。由于使用峰移动而不是峰强度进行定性分析，因此SERS底物的等离子特性和重现性问题不会很明显。

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