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Simultaneous observation of ultrafast ligand dissociation and docking-site trapping in heme proteins using upconversion infrared spectroscopy

机译:使用上转换红外光谱同时观察血红蛋白中超快的配体解离和对接位点捕获

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We report on ultrafast visible pump/mid-infrared probe spectroscopy of the carboxy form of heme proteins by employing the recently developed chirped-pulse upconversion technique, which allows both high resolution and sensitivity over an extremely broad spectral range. Commonly, the bleach signal due to ligand dissociation and the incipient docking-site absorption signal, being about 200 cm"1 apart and differing by more than an order of magnitude in absorptivity, are studied in separate experiments. We here monitor them simultaneously, allowing a direct observation and a concurrent analysis of the initial processes after photoinduced ligand dissociation, for instance, the formation of hot vibrational bands.
机译:我们通过使用最近开发的chi脉冲上转换技术报告血红素蛋白羧基形式的超快速可见泵浦/中红外探针光谱,该光谱在极宽的光谱范围内具有高分辨率和高灵敏度。通常,由于配体解离而产生的漂白信号和相距约200 cm“ 1且吸收率差异超过一个数量级的初期对接位点吸收信号。在单独的实验中进行了研究。直接观察并同时分析光诱导配体解离后的初始过程,例如热振动带的形成。

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