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Chapter 6 Layer-by-Layer Assembly of Enzymes on Carbon Nanotubes

机译：第6章碳纳米管上酶的逐层组装

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摘要

The use of layer-by-layer techniques for immobilizing several types of enzymes, e.g., glucose oxidase, horse radish oxidases, and choline oxidase, on carbon nanotubes (CNTs) and the applications of these techniques for biosensing are presented. The enzyme is immobilized on the negatively charged CNT surface by alternatively assembling a cationic polydiallyl-dimethyl-ammonium chloride (PDDA) layer and an enzyme layer. The sandwich-like layer structure (PDDA/enzyme/ PDDA/CNT) formed by electrostatic assembly provides a favorable microenvironment to retain the bioactivity of the enzyme and to prevent enzyme molecule leakage. The morphologies and electrocatalytic activity of the resulting enzyme films were characterized using transmission electron microscopy and electrochemical techniques, respectively. It was found that these enzyme-based biosensors are very sensitive and selective for the detection of biomolecules, e.g., glucose, choline.
机译：提出了使用分层技术将几种类型的酶固定在碳纳米管（CNT）上的方法，例如葡萄糖氧化酶，辣根氧化酶和胆碱氧化酶，以及这些技术在生物传感中的应用。通过交替组装阳离子聚二烯丙基-二甲基氯化铵（PDDA）层和酶层，将酶固定在带负电荷的CNT表面上。通过静电组装形成的三明治状层结构（PDDA /酶/ PDDA / CNT）提供了有利的微环境，以保持酶的生物活性并防止酶分子泄漏。分别使用透射电子显微镜和电化学技术对所得酶膜的形貌和电催化活性进行了表征。已经发现，这些基于酶的生物传感器对于检测生物分子例如葡萄糖，胆碱非常敏感并且具有选择性。

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