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首页> 外文会议>Biomolecular catalysis : Nanoscale science and technology >Chapter 12 Nanoporous Sol-Gel Supports Enzymatic Hydrolysis of Chlorophyll in Organic Media
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Chapter 12 Nanoporous Sol-Gel Supports Enzymatic Hydrolysis of Chlorophyll in Organic Media

机译：第十二章纳米多孔溶胶凝胶支持有机介质中叶绿素的酶水解

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摘要

Entrapment of membrane proteins is a challenging task compared to that involving soluble proteins. Chlorophyllase, a membrane protein, was entrapped in nanoporous tetramethoxysilane (TMOS)-derived sol-gel, demonstrating higher protein mass and activity yield than that in polysaccharide gel and hybrid gel. Both sol-gel formulation process and chemistry may affect apparent activity of sol-gel entrapped chlorophyllase, thus need to be taken into account to improve entrapped chlorophyllase activity. Even though the external transfer of chlorophyll to the sol-gel matrix can be minimized by increasing mixing rate up to 150 rpm, internal diffusion of chlorophyll in nanoporous sol-gel was largely restricted, confirmed by the measured diffusion coefficient of 10~(-14) m~2/s. The apparent activity was also affected by the availability of hydrophobic chlorophyll substrate within the hydrophilic gel, which was controlled by partitioning of chlorophyll between a water/organic solvent medium and the gel phase.
机译：与涉及可溶性蛋白的捕获相比，捕获膜蛋白是一项艰巨的任务。叶绿素酶是一种膜蛋白，被截留在纳米多孔四甲氧基硅烷（TMOS）衍生的溶胶-凝胶中，与多糖凝胶和杂化凝胶相比，具有更高的蛋白质质量和活性产率。溶胶-凝胶的配制过程和化学性质都可能影响溶胶-凝胶截留的叶绿素酶的表观活性，因此需要考虑改善包裹的叶绿素酶的活性。即使通过提高混合速度至150 rpm可以最大程度地减少叶绿素向溶胶-凝胶基质的外部转移，但叶绿素在纳米多孔溶胶-凝胶中的内部扩散受到很大的限制，这由测得的10〜（-14）扩散系数证实）m〜2 / s。表观活性还受到亲水性凝胶中疏水性叶绿素底物的可用性的影响，这是通过在水/有机溶剂介质和凝胶相之间分配叶绿素来控制的。

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  • 会议地点 Washington DC(US);Washington DC(US)
  • 作者

    Yunyu Yi; Selim Kermasha; Ronald Neufeld;

  • 作者单位

    Department of Chemical Engineering, Queen's University, Kingston, Ontario K7L 3N6, Canada;

    Department of Food Science and Agricultural Chemistry, McGill University, 21111 Lakeshore Road, Ste-Anne de Bellevue, Quebec H9X 3V9, Canada;

    Department of Chemical Engineering, Queen's University, Kingston, Ontario K7L 3N6, Canada;

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  • 正文语种 eng
  • 中图分类 工程材料一般性问题;
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