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Tumor redox metabolism correlation with the expression level of red fluorescent protein

机译：肿瘤氧化还原代谢与红色荧光蛋白表达水平的相关性

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摘要

The redox metabolism is variable and complicated with the progress of tumor development. Whether the tumor redox state will affect the exogenous gene expression or not, are still not clear now . To investigate the relationship between tumor endogenous redox state and the exogenous gene expression level, a far red fluorescent protein fRFP was used to monitor tumor cells proliferation and as an exogenous protein expression in tumors. NADH (nicotinamide adenine dinucleotide) and Fp (flavin protein) are two important coenzymes in the mitochondria respiratory chain, which can be as a standard representation for redox metabolism state. Three tumor subcutaneous models (melanoma, human pancreatic carcinoma and nasopharyngeal carcinoma) were used to observe their redox state and protein expression by our home-made redox scanner. The results showed that the distribution of fRFP fluorescent protein expression in the inner tumor regions are heterogeneous, and the fluorescent intensity of fRFP and the fluorescent intensity of NADH have high correlation, In addition, we also found the linear coefficient in three tumors are different, the value of coefficient is (R2 = 0.966 and R2 = 0.943) in melanoma, (R2 = 0.701 and R2 = 0.942) in human pancreatic carcinoma, and (R2 = 0.994) in nasopharyngeal carcinoma, respectively. From these results, we consider that the exogenous protein expression of fRFP in tumor had some relationship with the tumor redox state of NADH.
机译：氧化还原代谢是可变的，并且随着肿瘤的发展而复杂化。现在尚不清楚肿瘤的氧化还原状态是​​否会影响外源基因的表达。为了研究肿瘤内源性氧化还原状态与外源基因表达水平之间的关系，使用了远红色荧光蛋白fRFP来监测肿瘤细胞的增殖并作为肿瘤中外源蛋白的表达。 NADH（烟酰胺腺嘌呤二核苷酸）和Fp（黄素蛋白）是线粒体呼吸链中的两个重要辅酶，可以作为氧化还原代谢状态的标准表示。通过我们自制的氧化还原扫描仪，使用三种肿瘤皮下模型（黑色素瘤，人胰腺癌和鼻咽癌）观察其氧化还原状态和蛋白质表达。结果表明，肿瘤内部fRFP荧光蛋白表达的分布是异质的，fRFP的荧光强度与NADH的荧光强度有较高的相关性，此外，我们还发现三种肿瘤的线性系数不同，在黑色素瘤中，系数值分别为（R2 = 0.966和R2 = 0.943），在人胰腺癌中分别为（R2 = 0.701和R2 = 0.942），在鼻咽癌中分别为（R2 = 0.994）。从这些结果，我们认为fRFP在肿瘤中的外源蛋白表达与NADH的肿瘤氧化还原状态有一定关系。

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  • 来源
    《Biophotonics and immune responses X》|2015年|932411.1-932411.5|共5页
  • 会议地点 San Francisco CA(US)
  • 作者

    Shuang Sha; Anle Wang; Qiaoya Lin; Zhihong Zhang;

  • 作者单位

    Britton Chance Center for Biomedical Photonics-Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, P.R. China;

    Britton Chance Center for Biomedical Photonics-Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, P.R. China;

    Britton Chance Center for Biomedical Photonics-Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, P.R. China;

    Britton Chance Center for Biomedical Photonics-Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, P.R. China;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Redox ratio; Fluorescent protein; KatushkaS158A; Nasopharyngeal carcinoma; Melanoma; Human pancreatic carcinoma;

    机译：氧化还原比；荧光蛋白； KatushkaS158A;鼻咽癌；黑色素瘤人胰腺癌;

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