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ALA-PDT mediated DC vaccine for skin squamous cell carcinoma

机译：ALA-PDT介导的DC皮肤鳞状细胞癌疫苗

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摘要

Dendritic cell (DC) based vaccine has emerged as a promising immunotherapy for cancers. However, most DC vaccines so far have only achieved limited success in cancer treatment. Photodynamic therapy (PDT), an established cancer treatment strategy, can cause immunogenic apoptosis to induce an effective antitumor immune response. In this study, we developed a DC-based cancer vaccine using immunogenic apoptotic tumor cells induced by 5-aminolevulinic acid (ALA) mediated PDT. The maturation of DCs induced by PDT-treated apoptotic cells was evaluated. The anti-tumor immunity of ALA-PDT-DC vaccine was tested with mouse model. We observed the maturations of DCs potentiated by ALA-PDT treated tumor cells, including phenotypic maturation (upregulation of surface expression of MHC-Ⅱ, DC80, and CD86), and functional maturation (enhanced capability to secret INF-γ and IL-12). ALA-PDT-DC vaccine mediated by apoptotic cells provided protection against tumor in mice, far stronger than that of DC vaccine obtained from freeze/thaw treated tumor cells. Our results indicate that immunogenic apoptotic tumor cells can be more effective in enhancing DC-based cancer vaccine, which could improve the clinical application of PDT- DC vaccines.
机译：基于树突细胞（DC）的疫苗已成为一种有前途的癌症免疫疗法。但是，到目前为止，大多数DC疫苗在癌症治疗中仅取得了有限的成功。光动力疗法（PDT）是一种既定的癌症治疗策略，可以引起免疫原性细胞凋亡，从而诱导有效的抗肿瘤免疫反应。在这项研究中，我们使用5-氨基乙酰丙酸（ALA）介导的PDT诱导的免疫原性凋亡肿瘤细胞开发了一种基于DC的癌症疫苗。评估了由PDT处理的凋亡细胞诱导的DC成熟。用小鼠模型测试了ALA-PDT-DC疫苗的抗肿瘤免疫性。我们观察到ALA-PDT处理的肿瘤细胞增强的DC成熟，包括表型成熟（MHC-Ⅱ，DC80和CD86的表面表达上调）和功能成熟（增强了分泌INF-γ和IL-12的能力） 。由凋亡细胞介导的ALA-PDT-DC疫苗对小鼠的肿瘤具有保护作用，远强于从冻融处理的肿瘤细胞获得的DC疫苗。我们的结果表明，免疫原性凋亡肿瘤细胞在增强基于DC的癌症疫苗方面可以更有效，从而可以改善PDT-DC疫苗的临床应用。

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  • 来源
    《Biophotonics and immune responses X》|2015年|93240A.1-93240A.10|共10页
  • 会议地点 San Francisco CA(US)
  • 作者

    Jie Ji; Zhixia Fan; Feifan Zhou; Xiaojie Wang; Lei Shi; Haiyan Zhang; Peiru Wang; Degang Yang; Linglin Zhang; Xiuli Wang; Wei R. Chen;

  • 作者单位

    Shanghai Skin Disease Hospital, 1278 Baode Road, Shanghai 200443, China;

    Shanghai Skin Disease Hospital, 1278 Baode Road, Shanghai 200443, China;

    Biophotonics Research Laboratory, Center for Interdisciplinary Biomedical Education and Research, University of Central Oklahoma, Edmond, OK 73034, USA;

    Shanghai Skin Disease Hospital, 1278 Baode Road, Shanghai 200443, China;

    Shanghai Skin Disease Hospital, 1278 Baode Road, Shanghai 200443, China;

    Shanghai Skin Disease Hospital, 1278 Baode Road, Shanghai 200443, China;

    Shanghai Skin Disease Hospital, 1278 Baode Road, Shanghai 200443, China;

    Shanghai Skin Disease Hospital, 1278 Baode Road, Shanghai 200443, China;

    Shanghai Skin Disease Hospital, 1278 Baode Road, Shanghai 200443, China;

    Shanghai Skin Disease Hospital, 1278 Baode Road, Shanghai 200443, China;

    Biophotonics Research Laboratory, Center for Interdisciplinary Biomedical Education and Research, University of Central Oklahoma, Edmond, OK 73034, USA,Shanghai Skin Disease Hospital, 1278 Baode Road, Shanghai 200443, China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Dendritic cell; photodynamic therapy; immunogenic apoptosis; 5-aminolevulinic acid; PDT-DC vaccine;

    机译：树突状细胞；光动力疗法；免疫原性凋亡5-氨基乙酰丙酸; PDT-DC疫苗;

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