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The immunological response created by interstitial and non-invasive laser immunotherapy

机译:间质性和非侵入性激光免疫疗法产生的免疫反应

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摘要

Laser immunotherapy (LIT) is an innovative cancer modality that uses laser irradiation and immunological stimulation to treat late-stage, metastatic cancers. LIT can be performed through either interstitial or non-invasive laser irradiation. Although LIT is still in development, recent clinical trials have shown that it can be used to successfully treat patients with late-stage breast cancer and melanoma. The development of LIT has been focused on creating an optimal immune response created by irradiating the tumor. One important factor that could enhance the immune response is the duration of laser irradiation. Irradiating the tumor for a shorter or longer amount of time could weaken the immune response created by LIT. Another factor that could weaken this immune response is the proliferation of regulatory T cells (T_(Regs)) in response to the laser irradiation. However, low dose cyclophosphamide (CY) can help suppress the proliferation of T_(Regs) and help create a more optimal immune response. An additional factor that could weaken the effectiveness of LIT is the selectivity of the laser. If LIT is performed non-invasively, then deeply embedded tumors and highly pigmented skin could cause an uneven temperature distribution inside the tumor. To solve this problem, an immunologically modified carbon nanotube system was created by using an immunoadjuvant known as glycated chitosan (GC) as a surfactant for single-walled carbon nanotubes (SWNTs) to immunologically modify SWNTs. SWNT-GC retains the optical properties of SWNTs and the immunological functions of GC to help increase the selectivity of the laser and create a more optimal immune response. In this preliminary study, tumor-bearing rats were treated with LIT either interstitially by an 805-nm laser with GC and low-dose CY, or non-invasively by a 980-nm laser with SWNT-GC. The goal was to observe the effects of CY on the immune response induced by LIT and to also determine the effect of irradiation duration for interstitial and non-invasive LIT.
机译:激光免疫疗法(LIT)是一种创新的癌症形式,使用激光照射和免疫刺激治疗晚期转移性癌症。 LIT可以通过间质性或非侵入性激光照射进行。尽管LIT仍在开发中,但最近的临床试验表明,它可以成功地治疗晚期乳腺癌和黑色素瘤患者。 LIT的发展一直集中在通过照射肿瘤产生最佳免疫反应上。可以增强免疫反应的重要因素之一是激光照射的持续时间。照射肿瘤较短或更长时间可以减弱LIT产生的免疫反应。可能削弱这种免疫反应的另一个因素是响应激光照射,调节性T细胞(T_(Regs))的增殖。但是,低剂量环磷酰胺(CY)可以帮助抑制T_(Regs)的增殖,并有助于产生更理想的免疫反应。可能削弱LIT有效性的另一个因素是激光的选择性。如果以非侵入性方式进行LIT,则深层包埋的肿瘤和色素沉着的皮肤可能会导致肿瘤内部温度分布不均。为了解决此问题,通过使用称为糖化壳聚糖(GC)的免疫佐剂作为单壁碳纳米管(SWNT)的表面活性剂来免疫修饰SWNT,从而创建了一种免疫修饰的碳纳米管系统。 SWNT-GC保留了SWNTs的光学特性和GC的免疫功能,以帮助增加激光的选择性并产生更理想的免疫反应。在这项初步研究中,荷瘤大鼠用805 nm的GC和低剂量CY激光间质性地用LIT治疗,或者用980 nm的SWNT-GC激光无创地治疗。目的是观察CY对LIT诱导的免疫反应的影响,并确定间质性和非侵入性LIT的照射持续时间的影响。

著录项

  • 来源
    《Biophotonics and immune responses X》|2015年|93240D.1-93240D.8|共8页
  • 会议地点 San Francisco CA(US)
  • 作者单位

    Center for Interdisciplinary Biomedical Education and Research, University of Central Oklahoma, Edmond, OK 73034, USA,Biophotonics Research Laboratory, Department of Engineering and Physics, University of Central Oklahoma, Edmond, OK 73034, USA;

    Center for Interdisciplinary Biomedical Education and Research, University of Central Oklahoma, Edmond, OK 73034, USA,Biophotonics Research Laboratory, Department of Engineering and Physics, University of Central Oklahoma, Edmond, OK 73034, USA;

    Center for Interdisciplinary Biomedical Education and Research, University of Central Oklahoma, Edmond, OK 73034, USA,Biophotonics Research Laboratory, Department of Engineering and Physics, University of Central Oklahoma, Edmond, OK 73034, USA;

    Center for Interdisciplinary Biomedical Education and Research, University of Central Oklahoma, Edmond, OK 73034, USA,Biophotonics Research Laboratory, Department of Engineering and Physics, University of Central Oklahoma, Edmond, OK 73034, USA;

    Immunophotonics Inc., 4320 Forest Park Avenue #303, St. Louis, Missouri 63108, USA;

    Immunophotonics Inc., 4320 Forest Park Avenue #303, St. Louis, Missouri 63108, USA;

    Center for Bioengineering and School of Electrical and Computer Engineering, University of Oklahoma, Norman, Oklahoma, 73019, USA;

    Center for Interdisciplinary Biomedical Education and Research, University of Central Oklahoma, Edmond, OK 73034, USA,Biophotonics Research Laboratory, Department of Engineering and Physics, University of Central Oklahoma, Edmond, OK 73034, USA;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Single-walled carbon nanotubes; thermal cytotoxicity; interstitial laser irradiation; metastatic cancer; immunological stimulant; glycated chitosan; cyclophosphamide;

    机译:单壁碳纳米管;热细胞毒性间隙激光照射转移性癌症;免疫刺激剂糖化壳聚糖环磷酰胺;

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