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Measurements of Gold Nanoparticle Concentration with K-shell X-ray Fluorescence Spectrum

机译:用K壳X射线荧光光谱法测定纳米金的含量。

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摘要

In this study, the AuNP k-shell fluorescence spectra were measured by using a 100 mm long collimator to improve the detectability. The AuNPs were suspended in deionized water at different concentrations. The AuNP suspensions were excited by a micro focal spot x-ray tube with 130 kVp, 300 μA x-ray exposures. The emissions of the AuNP fluorescence were measured by a spectrometer located with an angle of 90 degrees with respect to the excitation beam. The fluorescence acquisition durations for each concentration mode were 3000 s. A 1.0 mm Pb filter and a 1 mm Al filter were utilized to optimize excitation beam and fluorescence emission, respectively. As a result, the k-shell fluorescence peaks, 66.99 keV and 68.80 keV of AuNP, were measured and observed in 0.1, 0.2, 0.4, 0.8, 1.0, 2.0, 4.0 mg/mL concentration modes. The linear relationship between the AuNP suspension concentrations and the number of photons of the fluorescence peaks were observed in the range of 0.1-4.0 mg/mL. The results of experimental measurements demonstrated up to 0.1 mg/mL (0.01 % in weight concentration) detectability.
机译:在这项研究中,通过使用100 mm长的准直仪来测量AuNP k壳荧光光谱,以提高可检测性。 AuNPs以不同浓度悬浮在去离子水中。 AuNP悬浮液由具有130 kVp,300μAX射线暴露量的微焦点X射线管激发。用相对于激发光束成90度角的光谱仪测量AuNP荧光的发射。每个浓度模式的荧光采集持续时间为3000 s。利用1.0 mm Pb滤光片和1 mm Al滤光片分别优化激发光束和荧光发射。结果,在0.1、0.2、0.4、0.8、1.0、2.0、4.0mg / mL浓度模式下测量并观察到k-壳荧光峰AuNP的66.99keV和68.80keV。在0.1-4.0mg / mL的范围内观察到AuNP悬浮液浓度与荧光峰的光子数之间的线性关系。实验测量结果表明,可检测度高达0.1 mg / mL(重量浓度为0.01%)。

著录项

  • 来源
    《Biophotonics and immune responses XII》|2017年|100650M.1-100650M.5|共5页
  • 会议地点 San Francisco(US)
  • 作者单位

    Center of Bioengineering and School of Electrical and Computer Engineering, University of Oklahoma;

    Center of Bioengineering and School of Electrical and Computer Engineering, University of Oklahoma;

    Center of Bioengineering and School of Electrical and Computer Engineering, University of Oklahoma;

    Center of Bioengineering and School of Electrical and Computer Engineering, University of Oklahoma;

    Center of Bioengineering and School of Electrical and Computer Engineering, University of Oklahoma;

    Department of Engineering and Physics, University of Central Oklahoma;

    Center of Bioengineering and School of Electrical and Computer Engineering, University of Oklahoma;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Gold nanoparticle; K-shell x-ray fluorescence; Concentration; Detectability;

    机译:金纳米粒子; K壳X射线荧光;浓度;可检测性;

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