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DEVELOPMENT OF BIOSENSORS FOR IMMUNOASSAY DETECTION OF PCB'S

机译：用于PCB免疫检测的生物传感器的开发

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摘要

Three different electrochemical approaches for the detection of naphthol (amperometry. chronoamperometry and differential pulse voltammetry). using screen-printed carbon electrodes, were compared with respect to measurable range, detection limit, reproducibility, sample throughput and suitability for in-field use. The highest sensitivity (252 nA/μM) with a calculated detection limit of 0.1 μM. combined with a remarkable 'time for sample measurement' (5.5 seconds) was achieved with differential pulse voltammetry. The standard error in the all measured range (0.5-100 μM) was below 5%. The scan speed and the pulse amplitude of the differential pulse voltammetry were optimised using the simplex method. The optimised electrochemical procedure was then used to evaluate the detection of low levels of alkaline phosphatase: this enzyme catalyses the hydrolysis of naphthyl phosphate to naphthol. Using 2 minutes as incubation time for the enzyme reaction, a calibration curve in the range 1-10~(-5) U/ml was obtained with a calculated detection limit of 2.1×10~(-6) U/ml. The standard error for the 10~(-2) U/ml enzyme standard was 6.8%. Finally a competitive enzyme immunoassay for polychlorinated biphenyls based on alkaline phosphatase was performed using the optimised electrochemical detection of naphthol. The assay measurable range was 0.01-10 μg/ml. and the detection limit was 0.01 μg/ml.
机译：三种检测萘酚的电化学方法（电流分析法，计时电流分析法和差分脉冲伏安法）。比较了使用丝网印刷碳电极的可测量范围，检测极限，重现性，样品通量和现场使用的适用性。最高灵敏度（252 nA /μM），计算出的检出限为0.1μM。差分脉冲伏安法可实现出色的“样品测量时间”（5.5秒）。所有测量范围（0.5-100μM）内的标准误差均低于5％。使用单纯形法优化了差分脉冲伏安法的扫描速度和脉冲幅度。然后将优化的电化学程序用于评估低水平碱性磷酸酶的检测：该酶催化磷酸萘酯水解为萘酚。使用2分钟作为酶反应的孵育时间，可获得1-10〜（-5）U / ml范围内的校准曲线，计算出的检出限为2.1×10〜（-6）U / ml。 10〜（-2）U / ml酶标的标准误为6.8％。最后，使用优化的萘酚电化学检测方法，对基于碱性磷酸酶的多氯联苯进行了竞争性酶免疫分析。该测定的可测量范围是0.01-10μg/ ml。检出限为0.01μg/ ml。

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  • 来源
  • 会议地点 Smolenice(SK)
  • 作者

    M. Del Carlo; I. Lionti; M. Taccini; A. Cagnini; M.Mascini;

  • 作者单位

    Dipartimento di Sanita Pubblica. Epidemiologia e Chimica Analitica Ambientale Sezione di Chimica Analitica. Universita di Firenze Via G.Capponi 9, 50121 Firenze, ITALIA;

    Dipartimento di Sanita Pubblica. Epidemiologia e Chimica Analitica Ambientale Sezione di Chimica Analitica. Universita di Firenze Via G.Capponi 9, 50121 Firenze, ITALIA;

    Dipartimento di Sanita Pubblica. Epidemiologia e Chimica Analitica Ambientale Sezione di Chimica Analitica. Universita di Firenze Via G.Capponi 9, 50121 Firenze, ITALIA;

    Dipartimento di Sanita Pubblica. Epidemiologia e Chimica Analitica Ambientale Sezione di Chimica Analitica. Universita di Firenze Via G.Capponi 9, 50121 Firenze, ITALIA;

    Dipartimento di Sanita Pubblica. Epidemiologia e Chimica Analitica Ambientale Sezione di Chimica Analitica. Universita di Fi;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 环境监测;
  • 关键词

    differential pulse voltammetry; immunoassay; alkaline phosphatase; screen-printed electrode;

    机译：差分脉冲伏安法免疫测定碱性磷酸酶丝网印刷电极;

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