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IMMUNOCHEMICAL ASSAYS FOR PESTICIDES AND PCBs

机译：农药和PCB的免疫化学测定

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Analytical parameters of any immunochemical assay are based primarily on binding characteristics of the antibody employed. In this laboratory, a wide variety of immunogens, antibodies and tracer conjugates have been developed to incorporate them into competitive enzyme linked immunoadsorbent assays (ELISAs) and other immunochemical formats including biosensors. The antibody based assays, working on competitive principle, provided sensitive responses to the presence of ppt-ppb amounts of toxic substances measured in terms of changes of radioactive, color, fluorescent, or electric signals. Thus, a sensitive and highly selective immunochemical analysis of 2,4-dichlorophenoxyacetic acid (2,4-D), s-triazines and polychlorinated biphenyls (PCBs) can be performed under both laboratory and field conditions. Favorable correlation between immunochemical and instrumental techniques has been found. Several interrelated issues concerning the conventional immunoassays and immuno-sensors are compared and discussed in terms of needs and methodological maturation.
机译：任何免疫化学测定的分析参数主要基于所用抗体的结合特性。在这个实验室中，已经开发了各种各样的免疫原，抗体和示踪结合物，以将它们掺入竞争性酶联免疫吸附测定（ELISA）和其他免疫化学形式，包括生物传感器。基于竞争性原理的基于抗体的测定，对放射性，颜色，荧光或电信号变化测量的ppt-ppb量有毒物质的存在提供了敏感的响应。因此，可以在实验室和现场条件下对2，4-二氯苯氧基乙酸（2,4-D），S-三嗪和多氯联苯（PCB）进行灵敏且高度选择性的免疫化学分析。已经发现免疫化学和仪器技术之间的良好相关性。根据需求和方法学的成熟度，对与常规免疫测定法和免疫传感器有关的几个相互关联的问题进行了比较和讨论。

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