DETERMINATION OF HEAVY METALS USING ELECTROCHEMICAL BIOSENSORS BASED ON ENZYME INHIBITION

机译：基于酶抑制的电化学生物传感器测定重金属

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A bioelectrochemical method for the determination of heavy metals has been developed using oxidase enzymes and screen-printed electrodes. The activity of the oxidase enzymes has been measured following H_2O_2 production in the presence of the substrate with a Ru/graphite working electrode polarized at +700 mV vs. Ag/AgCl. Interfence signals due to the oxidation of the enzyme substrates have been minimized by the use of a cellulose acetate membrane placed onto the electrode surface. Inhibition of the activity of enzymes such as alcohol oxidase, sarcosine oxidase and glycerol-3-P oxidase by the metal ions Hg(Ⅱ), V(Ⅴ), Cu(Ⅱ), Ni(Ⅱ) resulted in the costruction of calibration curves for the metals in the low ppm range. Total analysis time was 10 min (5 min incubation + 5 min measuring) with CVs of 10-17 %.

机译：已经开发出一种使用氧化酶和丝网印刷电极测定重金属的生物电化学方法。在底物存在下，在Ru /石墨工作电极相对于Ag / AgCl极化为+700 mV的情况下，在产生H_2O_2之后，测量了氧化酶的活性。通过使用置于电极表面的醋酸纤维素膜，已将由于酶底物氧化引起的干扰信号降至最低。金属离子Hg（Ⅱ），V（Ⅴ），Cu（Ⅱ），Ni（Ⅱ）对醇氧化酶，肌氨酸氧化酶和甘油-3-P氧化酶等酶活性的抑制作用导致校正曲线的建立对于低ppm范围的金属。总分析时间为10分钟（孵育5分钟+测量5分钟），CV为10-17％。

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来源
《Biosensors for direct monitoring of environmental pollutants in field》|1997年|220-226|共7页
会议地点 Smolenice(SK)
作者
D. COMPAGNONE; M. BUGLI; P. IMPERIALI; G. VARALLO; G. PALLESCHI;
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Dipartimento di Scienze e Tecnologie Chimiche, Universita' di Roma 'Tor Vergata', via della Ricerca Scientifica, 00133 Rome, Italy;

Dipartimento di Scienze e Tecnologie Chimiche, Universita' di Roma 'Tor Vergata', via della Ricerca Scientifica, 00133 Rome, Italy;

ISMES S.pA., viale Giulio Cesare 29, 24100 Bergamo. Italy;

ENEL, Direzione Studi e Ricerche, Certtro Valorizzazione e Trattamento Residui, via Dalmazia 21/C, 72100 Brindisi, Italy;

Dipartimento di Scienze e Tecnologie Chimiche, Universita' di Roma 'Tor Vergata', via della Ricerca Scientifica, 00133 Rome, Italy;

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正文语种 eng
中图分类环境监测;
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DETERMINATION OF HEAVY METALS USING ELECTROCHEMICAL BIOSENSORS BASED ON ENZYME INHIBITION

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