首页> 外文会议>Biosensors for direct monitoring of environmental pollutants in field >USE OF A CCD-BASED IMAGING DETECTOR AND CAPILLARY SAMPLING TECHNIQUES FOR ENVIRONMENTAL TESTING
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USE OF A CCD-BASED IMAGING DETECTOR AND CAPILLARY SAMPLING TECHNIQUES FOR ENVIRONMENTAL TESTING

机译:使用基于CCD的成像检测器和毛细管采样技术进行环境测试

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A microformat ELISA has been developed for the simultaneous determination of multiple 2,4-dichlorophenoxyacetic acid (2,4-D) samples using a CCD-camera and highly efficient chemiluminescent enzyme substrates. The linear range was 4.5×10~(-10) - 4.5×10~(-7) M with a lower detection limit of 4.3×10~(-10) M, corresponding to 96 pg/ml or 192 pg/well with a CV of 12.5%. The use of gold coated silicon wafers and glass capillaries as solid phase supports in the imaging ELISA is also described. The highly reflective gold surfaces improved the linear range and the sensitivity as compared to thick-film patterned surfaces. Sampling in capillary tubes reduces the sensitivity and the linear range, but there are indications that the capillaries can act as light guides and thereby improve the light collection efficiency.
机译:已开发出一种微格式ELISA,用于使用CCD相机和高效化学发光酶底物同时测定多个2,4-二氯苯氧基乙酸(2,4-D)样品。线性范围为4.5×10〜(-10)-4.5×10〜(-7)M,检测下限为4.3×10〜(-10)M,对应于96 pg / ml或192 pg /孔。 CV为12.5%。还描述了在成像ELISA中使用镀金的硅片和玻璃毛细管作为固相支持物。与厚膜图案化的表面相比,高反射率的金表面改善了线性范围和灵敏度。在毛细管中取​​样会降低灵敏度和线性范围,但是有迹象表明毛细管可以充当光导,从而提高了光收集效率。

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