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首页> 外文会议>Conference on advancing manufacture of cell and gene therapies >CHARACTERIZATION OF CAR-T TRANSDUCTION PARAMETERS USING A LENTIVIRAL VECTOR
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CHARACTERIZATION OF CAR-T TRANSDUCTION PARAMETERS USING A LENTIVIRAL VECTOR

机译：使用慢病毒载体表征CAR-T传递参数

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Production of chimeric antigen receptor T cells (CAR-T) can be accomplished using a viral vector to deliver the transgene. Lentiviral vectors (LVV) are commonly used for transduction due to their safety profile, stable transduction, ease of use, and ability to transduce both dividing and nondividing cells. Characterization studies were conducted to identify parameters within the manufacturing process that could impact transduction efficiency, such as the multiplicity of infection (MOI), application time after T cell activation, and LW degradation profile. Optimizing these parameters will result in improved reproducibility within the manufacturing process and the efficient use of LW material. Response curves for transduction efficiency, vector copy number and potency exhibited similar patterns to one another; signals increased with increasing MOI until reaching a plateau.
机译：嵌合抗原受体T细胞（CAR-T）的产生可以使用病毒载体来递送转基因来完成。慢病毒载体（LVV）由于其安全性，稳定的转导，易用性以及转导分裂和非分裂细胞的能力而通常用于转导。进行了表征研究，以确定制造过程中可能影响转导效率的参数，例如感染复数（MOI），T细胞活化后的应用时间以及LW降解曲线。优化这些参数将提高制造过程中的可重复性，并有效利用LW材料。转导效率，载体拷贝数和效价的响应曲线呈现出相似的模式。信号随着MOI的增加而增加，直到达到平稳状态。

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