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首页> 外文会议>Clinical Diagnostic Systems: Technologies and Instrumentation >Multiparametric Analysis of Fine Needle Aspirate Biopsies from Parotid Tumors by Laser Scanning Cytometry (LSC)
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Multiparametric Analysis of Fine Needle Aspirate Biopsies from Parotid Tumors by Laser Scanning Cytometry (LSC)

机译：腮腺肿瘤细针穿刺活检的多参数分析，通过激光扫描细胞术（LSC）

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摘要

In order to minimize hospitalization and morbidity with optimized therapy for a patient with a tumor of the parotid gland a malignancy must be confirmed or excluded as soon as possible. Up to now, non- and minimal-invasive methods do not yield this information. For fine needle aspirate biopsies (FNABs), analysis by a specialized cytologist yields subjective and qualitative but not objective and quantitative data. LSC is a semi-automated microscope-based technology and offers ideal prerequisites for the analysis of specimens fixed on a slide. We have established an assay for FNABs from parotid gland tumors. Cells are stained for cytokeratin and DNA. The analysis quantitatively determines the ploidy of the cells and the degree of condensation of the DNA; on this basis the percentage of cells undergoing mitosis can be determined. Subsequently the cells are stained by H&E and are re-localized on the slide at their fixed position. Micrographs are taken for objective documentation of the cells' morphology. Using this assay FNABs from parotid gland tumors were analyzed; tumors that were diagnosed as benign by routine histopathology showed no aneuploidy whereas malignant tumors were aneuploid. This preliminary study demonstrates the capacities of LSC for minimal-invasive assays yielding quantitative and objective data.
机译：为了使腮腺肿瘤患者的优化治疗使住院和发病率降至最低，必须尽快确认或排除恶性肿瘤。到目前为止，非侵入性和微创方法尚无法获得此信息。对于细针穿刺活检（FNAB），由专门的细胞学家进行分析可获得主观和定性的数据，而不是客观和定量的数据。 LSC是基于半自动显微镜的技术，为分析固定在载玻片上的标本提供了理想的先决条件。我们已经建立了腮腺肿瘤FNAB的检测方法。对细胞进行细胞角蛋白和DNA染色。该分析定量确定了细胞的倍性和DNA的缩合程度。在此基础上，可以确定有丝分裂细胞的百分比。随后，将细胞用H＆E染色，并在玻片上的固定位置重新定位。显微照片用于客观记录细胞的形态。使用该试验分析腮腺肿瘤的FNAB。通过常规组织病理学诊断为良性的肿瘤没有非整倍性，而恶性肿瘤是非整倍性。这项初步研究证明了LSC用于微创检测的能力，可产生定量和客观的数据。

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