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Optical size determination of quantum dots using FRET with terbium complexes as donors

机译:使用F配合物作为供体的FRET测定量子点的光学尺寸

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We present the use of luminescent terbium complexes (LTCs) as FRET donors and luminescent semiconductor quantum dots (QDs) as FRET acceptors for spectroscopie ruler measurements. The LTCs were labeled to polyhistidine-appended peptides which self-assembled onto three different QDs allowing optical multiplexed measurements. Forster distances were in the range of 60-75 A and FRET efficiencies of up to 97 % were realized. Time-resolved analysis allowed the determination of donor-acceptor separation distances. The results suggests the efficient use of our LTC-to-QD FRET systems for multiplexed optical size determination, molecular ruler measurements and multi-parameter diagnostics.
机译:我们目前使用发光ter络合物(LTC)作为FRET供体,使用发光半导体量子点(QD)作为FRET受体进行光谱尺测量。将LTC标记为附加有多组氨酸的肽,这些肽会自组装到三个不同的QD上,从而可以进行光学多路复用测量。福斯特距离在60-75 A之间,FRET效率高达97%。时间分辨分析允许确定供体-受体间隔距离。结果表明,将我们的LTC-to-QD FRET系统有效地用于多重光学尺寸确定,分子尺测量和多参数诊断。

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