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首页> 外文会议>Colloidal quantum dots for biomedical applications V >Energy transfer from terbium complexes to quantum dots - The advantage of independent donor and acceptor decay time analysis for investigations on FRET distance dependence
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Energy transfer from terbium complexes to quantum dots - The advantage of independent donor and acceptor decay time analysis for investigations on FRET distance dependence

机译：从ter配合物到量子点的能量转移-独立的供体和受体衰变时间分析在研究FRET距离依赖性方面的优势

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摘要

The efficient use of luminescent semiconductor quantum dots (QDs) as Forster Resonance Energy Transfer (FRET) acceptors can be accomplished with terbium complexes (TCs) as donors. TCs exhibit long excited state lifetimes (in the millisecond range) up to 105 times longer than typical QD lifetimes. When FRET occurs from TCs to QDs the measured TC luminescence decay times decrease (FRET quenching), whereas the QD decay times increase (FRET sensitization). Due to the large difference between the TC and QD excited state lifetimes the FRET formalism can be applied to both the TC donors as well as the QD acceptors. This is a big advantage because the FRET information from one experiment can be received from both sides of the FRET pair allowing for the use of different detection channels and wavelengths for donor and acceptor. Thus, a multiplexing format becomes possible with one single donor (TC) and several different acceptors (different QDs). In this contribution we show the theoretical background for simultaneously applying FRET to donor and acceptor and give an example with a commercially available TC-QD donor-acceptor pair.
机译：发光半导体量子点（QD）作为Forster共振能量转移（FRET）受体的有效利用可以通过ter络合物（TC）作为施主来实现。 TC具有长的激发态寿命（在毫秒范围内），是典型QD寿命的105倍。当从TC到QD发生FRET时，测得的TC发光衰减时间减少（FRET猝灭），而QD衰减时间增加（FRET敏化）。由于TC和QD激发态寿命之间的巨大差异，因此FRET形式主义可同时应用于TC供体和QD受体。这是一个很大的优势，因为可以从FRET对的两侧接收来自一个实验的FRET信息，从而允许对供体和受体使用不同的检测通道和波长。因此，使用一个单个供体（TC）和几个不同的受体（不同的QD）就可以实现多路复用格式。在这项贡献中，我们展示了同时将FRET应用于施主和受主的理论背景，并给出了一个可商购的TC-QD施主-受主的例子。

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