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Development of a two-photon polymerization and optical tweezers microscope for fabrication and manipulation of microstructures

机译：开发用于制造和操纵微结构的双光子聚合和光学镊子显微镜

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We report development of a two-photon polymerization (TPP) microscope, for micro-fabrication of microstructures, which is capable of optical manipulation by use of optical tweezers. The system is based on an inverted Nikon microscope with a tunable Ti: Sapphire femto-second (fs) laser coupled to the upper back port. While in mode-locked condition, nanoparticles and wires were fabricated in photo-polymerizable synthetic materials using TPP. By axial positioning of the focused TPP laser beam, ID-structures (for use as wave guide) were fabricated at desired height above the surface of the substrate. In the mode lock-OFF condition the same tunable laser microbeam was employed as optical tweezers to the hold the nanostructures and manipulate them even in highly viscous medium before immobilizing. Size of the TPP induced structure was found to depend on the fs laser intensity and exposure. Further, by shaping the fs laser beam to line pattern, linear ID structures could be fabricated without scanning the beam or stage, which remain aligned along the line intensity profile due to anisotropic trapping force of the line tweezers in X and Y-directions. Use of optical tweezers with two-photon polymerization not only allowed in-situ corrective positioning of the polymerized structures, but also the integration of fluorescent microspheres (resonator/detector) with polymerized waveguide.
机译：我们报告开发的两光子聚合（TPP）显微镜，用于微细加工的微结构，能够通过使用光镊进行光学操作。该系统基于一台尼康倒置显微镜，该显微镜具有可调谐的Ti：蓝宝石飞秒（fs）激光器，该激光器耦合至上后端口。在锁模状态下，使用TPP在可光聚合的合成材料中制造纳米颗粒和金属丝。通过轴向聚焦的TPP激光束进行定位，可以在基板表面上方的所需高度处制造ID结构（用作波导）。在模式锁定条件下，将相同的可调谐激光微束用作光镊，以固定纳米结构并在固定之前甚至在高粘度介质中对其进行操作。发现TPP诱导的结构的尺寸取决于fs激光强度和曝光。此外，通过将fs激光束整形为线形图案，可以制造线性ID结构而无需扫描光束或镜台，由于线形镊子在X和Y方向上的各向异性捕获力，其沿线强度轮廓保持对齐。将光镊与双光子聚合一起使用，不仅可以对聚合结构进行原位校正定位，而且还可以将荧光微球（谐振器/检测器）与聚合波导集成在一起。

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