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首页> 外文会议>Conference on Active and passive smart structures and integrated systems; 20090309-12; San Diego, CA(US) >Incorporation and characterization of biological molecules in droplet-interface bilayer networks for novel active systems
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Incorporation and characterization of biological molecules in droplet-interface bilayer networks for novel active systems

机译：新型主动系统的液滴界面双层网络中生物分子的掺入和表征

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摘要

Biological molecules including phospholipids and proteins offer scientists and engineers a diverse selection of materials to develop new types of active materials and smart systems based on ion conduction. The inherent energy-coupling abilities of these components create novel kinds of transduction elements. Networks formed from droplet-interface bilayers (DIB) are a promising construct for creating cell mimics that allow for the assembly and study of these active biological molecules. The current-voltage relationship of symmetric, "lipid-in" droplet-interface bilayers are characterized using electrical impedance spectroscopy (EIS) and cyclic voltammetry (CV). "Lipid-in" diphytanoyl phosphatidylcholine (DPhPC) droplet-interface bilayers have specific resistances of nearly 10MΩ·cm~2 and rupture at applied potentials greater than 300mV, indicating the "lipid-in" approach produces higher quality interfacial membranes than created using the original "lipid-out" method. The incorporation of phospholipids into the droplet interior allows for faster monolayer formation but does not inhibit the self-insertion of transmembrane proteins into bilayer interfaces that separate adjacent droplets. Alamethicin proteins inserted into single and multi-DIB networks produce a voltage-dependent membrane conductance and current measurements on bilayers containing this type of protein exhibit a reversible, 3-4 order-of-magnitude conductance increase upon application of voltage.
机译：包括磷脂和蛋白质在内的生物分子为科学家和工程师提供了多种选择的材料，以开发新型的活性材料和基于离子传导的智能系统。这些组件固有的能量耦合能力创造了新型的转导元件。由液滴界面双层（DIB）形成的网络是创建细胞模拟物的有前途的构建体，该模拟物允许组装和研究这些活性生物分子。使用电阻抗光谱（EIS）和循环伏安法（CV）表征对称的“脂质进入”液滴界面双层的电流-电压关系。 “ Lipid-in”二植烷酰基磷脂酰胆碱（DPhPC）液滴界面双层具有近10MΩ·cm〜2的比电阻，在大于300mV的施加电势下会破裂，这表明“ Lipid-in”方法产生的质量比使用Lipo-in的方法高。原始的“掉脂”方法。将磷脂掺入液滴内部允许更快的单层形成，但不会抑制跨膜蛋白自插入到分隔相邻液滴的双层界面中。插入到单DIB和多DIB网络中的Alamethicin蛋白会产生电压依赖性膜电导，并且在施加此电压后，包含此类蛋白的双层的电流测量结果显示可逆的3-4数量级电导增加。

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