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首页> 外文会议>Conference on Biomedical Applications of Micro-and Nanoengineering, Dec 16-18, 2002, Melbourne, Australia >Amplification and modelling of bioaffinity detection with terahertz spectroscopy
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Amplification and modelling of bioaffinity detection with terahertz spectroscopy

机译：太赫兹光谱生物亲和力检测的放大和建模

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摘要

A new bioaffinity sensor based on pulsed terahertz (THz) spectroscopy is able to sensitively detect the presence of ultra-thin bound biomolecular layers. The protein avidin and lipid biotin are noted for their very high binding affinity, and the ease for which they can be attached to residues with importance in many biosensing applications. We demonstrate the sensitivity of the pulsed THz spectrometer to thin avidin layers and to avidin amplified with micron-sized agarose beads. The experimental results can be simply modelled by considering transmission of the THz radiation at the thin film interfaces. We detect less than 10.3 ng/cm~2 avidin, giving the THz system a detection capability of sub-thin solid films better than ellipsometry and reflectometry techniques.
机译：一种基于脉冲太赫兹（THz）光谱的新型生物亲和力传感器能够灵敏地检测超薄结合的生物分子层的存在。亲和素蛋白和脂质生物素蛋白的结合亲和力非常高，在许多生物传感应用中，它们易于与残基连接的重要性也很明显。我们证明了脉冲THz光谱仪对薄的抗生物素蛋白层和用微米级琼脂糖珠放大的抗生物素蛋白的敏感性。通过考虑薄膜界面处THz辐射的传输，可以简单地对实验结果进行建模。我们检测到不到10.3 ng / cm〜2的抗生物素蛋白，使THz系统的亚薄固体膜检测能力优于椭偏和反射法。

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