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首页> 外文会议>Conference on biomedical applications of micro- and nanoengineering IV and complex systems; 20081210-12; Melbourne(AU) >Comparative study on functionalized SB A-15 and SBA-16 nanostructured materials used for immobilization of D-amino acid oxidase
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Comparative study on functionalized SB A-15 and SBA-16 nanostructured materials used for immobilization of D-amino acid oxidase

机译：用于固定化D-氨基酸氧化酶的功能化SB A-15和SBA-16纳米结构材料的比较研究

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摘要

SBA-15 and SBA-16 nanostucrured materials were synthesized via hydrothermal treatment and were functionalized with 3-aminopropyltriethoxysilane (APTES), and vinyltriethoxysilane (VTES) The obtained samples were characterized by different techniques such as XRD, BET, IR and TEM. After functionalization, it showed that these nanostrucrured materials were still maintained the hexagonal pore structure of the parent SBA-15 and cubic cage structure of the parent SBA-16. The non-functionalized pure silica SBA-15 and SBA-16 as well as functionalized SBA-15 and SBA-16 materials were used to immobilize DAAO, which is industrially important enzyme for the production of glutaryl 7-amino cephalosporanic acid (GL-7-ACA) from cephalosporin C (CPC). The obtained results revealed that functionalized SBA-15 and SBA-16 materials exhibited higher enzymatic activity and stability than those of non-functionalized ones. This might be due to the enhancing of surface hydrophobicity upon functionalization. The surface functionalization of the nanostructured silicas with organic groups can enhance the interaction between enzyme and the supports and consequently increasing the operational stability of the immobilized enzyme. The loading of enzyme on SBA-15 materials was higher than that on SBA-16 samples (both functionalized and non-functionalized types). This might be explained by the difference in pore size and type (cylindrical for SBA-15 and bottle-neck for SBA-16) as well as structure shape (hexagonal for SBA-15 and cubic cage for SBA-16) of both mesoporous materials. Additionally, nature of functionalized groups significantly affected the enzymatic activity. Effects on surface binding force, nature of functional groups, pore size of supports were investigated and discussed.
机译：通过水热处理合成了SBA-15和SBA-16纳米材料，并用3-氨基丙基三乙氧基硅烷（APTES）和乙烯基三乙氧基硅烷（VTES）进行了功能化。所获得的样品通过XRD，BET，IR和TEM等不同技术进行了表征。官能化后，表明这些纳米结构材料仍保持了母体SBA-15的六角孔结构和母体SBA-16的立方笼结构。非功能化的纯二氧化硅SBA-15和SBA-16以及功能化的SBA-15和SBA-16材料用于固定DAAO，DAAO是生产戊二酰7-氨基头孢烷酸（GL-7）的工业重要酶-ACA）来自头孢菌素C（CPC）。所得结果表明，功能化的SBA-15和SBA-16材料比非功能化的材料具有更高的酶促活性和稳定性。这可能是由于官能化后表面疏水性的增强。具有有机基团的纳米结构二氧化硅的表面官能化可增强酶与载体之间的相互作用，并因此增加固定化酶的操作稳定性。 SBA-15材料上的酶载量高于SBA-16样品（功能化和非功能化类型）上的酶载量。两种中孔材料的孔径和类型（SBA-15为圆柱形，SBA-16为瓶颈）以及结构形状（SBA-15为六角形，SBA-16为立方笼）的差异可以解释。另外，官能团的性质显着影响酶的活性。研究和讨论了对表面结合力，官能团性质，载体孔径的影响。

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