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首页> 外文会议>Conference on Environmental Pollution and Public Health >Origin and Spread Identification of Escherichia Coli Aerosol of Swine House Surroundings Using ERIC-PCR and REP-PCR
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Origin and Spread Identification of Escherichia Coli Aerosol of Swine House Surroundings Using ERIC-PCR and REP-PCR

机译：利用ERIC-PCR和REP-PCR鉴定猪舍环境中大肠杆菌气溶胶的来源和传播

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摘要

Evidence is mounting that microorganisms originating from livestock impact the air quality of the animal houses themselves and contribute to their neighborhood of the public.The aim of this study was to develop efficient bacterial source tracking capabilities to identify sources of Escherichia coli aerosol pollution caused by pigs.Airbome E.coli were isolated from indoor air,upwind air (10 and 50 m away) and downwind air samples (10,50,100,200 and 400 m away) for five swine houses using six-stage Andersen microbial samplers and RCS (Reuter-Centrifugal samplers).E.coli strains from pig fecal samples were also collected simultaneously.The enterobacterial repetitive intergenic consensus polymerise chain reaction (ERIC-PCR) and the repetitive extragenic palindromic (REP-PCR) approaches were used to study the genetic variability and to determine the strain relationships among E.coli isolated from different sites in each swine house.Results showed that 35.1％ (20/57) of the bacterial DNA fingerprints from the fecal isolates matched with the corresponding strains isolated from indoor and downwind air samples (similarity ≥ 90％).E.coli strains from the indoor and downwind air samples were closely related to the E.coli strains isolated from feces,while those isolated from upwind air samples (house C) had low similarity (61％-69％).Our results suggest that some strains isolated from downwind and indoor air originated in the swine feces.Effective hygienic measures should be taken in animal farms to prevent or minimize the downwind spread of microorganism aerosol.
机译：越来越多的证据表明，源自家畜的微生物会影响动物房屋本身的空气质量，并影响其周围的公众。这项研究的目的是开发有效的细菌源追踪功能，以识别猪造成的大肠杆菌气溶胶污染源使用六级安徒生微生物采样器和RCS（罗伊特离心）技术从五种猪舍的室内空气，迎风空气（距离10和50 m）和顺风空气样品（距离10,50,100,200和400 m）中分离出大肠杆菌同时收集猪粪样本中的大肠杆菌菌株，采用肠细菌重复基因间共识聚合链反应（ERIC-PCR）和重复性外源回文（REP-PCR）方法研究遗传变异性并确定结果表明，每只猪舍内不同部位分离的大肠杆菌之间具有菌株关系。结果表明，细菌DNA中有35.1％（20/57）呈环状。粪便分离株的指纹图谱与从室内和顺风样品中分离出的相应菌株相匹配（相似度≥90％）。室内和顺风样品中的大肠杆菌菌株与从粪便中分离出的大肠杆菌密切相关。从上风空气样本（C室）中分离出的相似度很低（61％-69％）。我们的结果表明，从下风和室内空气中分离出的某些菌株起源于猪的粪便。应在动物农场采取有效的卫生措施以预防或预防尽量减少微生物气溶胶的顺风传播。

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  • 会议地点 Shanghai(CN)
  • 作者

    Huiyong Duan; Tongjie Chai; Dunjiang Liu;

  • 作者单位

    College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Sino-German Cooperative Research Centre for Zoonoses of Animal Origin of Shandong Province,Tai'an,China,271018 State Environmental Protection Key Laboratory of Microorganism Application and Risk Control,Tsinghua University,Beijing,China,100084;

    College of Animal Husbandry and Veterinary Medicine,JiningAgricultural School,Jining,Shandong,China;

    College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Sino-German Cooperative Research Centre for Zoonoses of Animal Origin of Shandong Province,Tai'an,China,271018 State Environmental Protection Key Laboratory of Microorganism Application and Risk Control,Tsinghua University,Beijing,China,100084;

    College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Sino-German Cooperative Research Centre for Zoonoses of Animal Origin of Shandong Province,Tai'an,China,271018 State Environmental Protection Key Laboratory of Microorganism Application and Risk Control,Tsinghua University,Beijing,China,100084;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 对人体的危害;
  • 关键词

    Source identification; Swine house; Airborne Escherichia coli; ERIC-PCR; REP-PCR;

    机译：来源鉴定;猪房;机载大肠杆菌; ERIC-PCR; REP-PCR;

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