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首页> 外文会议>Conference on Imaging, Manipulation, and Analysis of Biomolecules, Cell, and Tissues; 20080121-23; San Jose,CA(US) >The NANIVID: A New Device for Cancer Cell Migration Studies
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The NANIVID: A New Device for Cancer Cell Migration Studies

机译：NANIVID：用于癌细胞迁移研究的新设备

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摘要

Cancerous tumors are dynamic microenvironments that require unique analytical tools for their study. Better understanding of tumor microenvironments may reveal mechanisms behind tumor progression and generate new strategies for diagnostic marker development, which can be used routinely in histopathological analysis. Previous studies have shown that cell invasion and intravasation are related to metastatic potential and have linked these activities to gene expression patterns seen in migratory and invasive tumor cells in vivo. Existing analytical methods for tumor microenvironments include collection of tumor cells through a catheter needle loaded with a chemical or protein attractant (chemoattractant). This method has some limitations and restrictions, including time constraints of cell collection, long term anesthetization, and in vivo imaging inside the catheter. In this study, a novel implantable device was designed to replace the catheter-based method. The 1.5mm × 0.5mm × 0.24mm device is designed to controllably release chemoattractants for stimulation of tumor cell migration and subsequent cell capture. Devices were fabricated using standard microfabrication techniques and have been shown to mediate controlled release of bovine serum albumin (BSA) and epidermal growth factor (EGF). Optically transparent indium tin oxide (ITO) electrodes have been incorporated into the device for impedance-based measurement of cell density and have been shown to be compatible with in vivo multi-photon imaging of cell migration.
机译：癌性肿瘤是动态的微环境，需要独特的分析工具进行研究。对肿瘤微环境的更好理解可能揭示了肿瘤进展背后的机制，并产生了诊断标记物开发的新策略，可将其常规用于组织病理学分析。先前的研究表明，细胞的侵袭和浸润与转移潜能相关，并将这些活性与体内迁移和侵袭性肿瘤细胞中所见的基因表达模式相关联。现有的针对肿瘤微环境的分析方法包括通过装有化学或蛋白质引诱剂（chemoattractant）的导管针收集肿瘤细胞。该方法具有一些限制和限制，包括细胞收集的时间限制，长期麻醉和导管内的体内成像。在这项研究中，设计了一种新型的可植入设备来替代基于导管的方法。 1.5mm×0.5mm×0.24mm的设备旨在可控地释放趋化因子，以刺激肿瘤细胞迁移和随后的细胞捕获。使用标准的微细加工技术制造的器械已经显示出介导牛血清白蛋白（BSA）和表皮生长因子（EGF）的控制释放。光学透明的铟锡氧化物（ITO）电极已集成到设备中，用于基于阻抗的细胞密度测量，并且已证明与细胞迁移的体内多光子成像兼容。

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