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首页> 外文会议>Conference on Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II; 20040127-20040128; San Jose,CA; US >Active particle manipulation with four laser beams
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Active particle manipulation with four laser beams

机译：四个激光束的主动粒子操纵

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摘要

Trapping and active movement of the sample in a microscope is gently performed by applying optical forces. Optical tweezers, including the recently developed dynamic multiple-beam optical tweezers, have been shown to be indispensable tools in several fields. We demonstrate an optical trap that is formed by four laser beams. In our Differential Active Optical Manipulator (DAOM) the beams are arranged along the axes of a tetrahedron and are approximately collimated within the vicinity of their point of intersection. The DAOM makes use of a distinctive advantage of collimated light: in contrast to optical tweezers, this instrument allows movement of the confined particle over long distances without mechanical scanning. The beams' scattering forces superimpose in their common volume, and the total force (magnitude and direction) on the particle can be set by adjusting the beams' intensities. This can be done quickly and independently for each beam, allowing us to move a particle along arbitrary paths within a volume of about 1nl. Active feedback ensures that the sample can be held in place or transported to a specified position as desired. This allows focusing and lateral translation of the microscopic sample, which are common tasks in microscopy. During manipulation the particle is simultaneously observed from four sides. This opens new perspectives for 3D microscopy combined with contactless manipulation of the sample under observation.
机译：通过施加光学力轻轻地进行样品在显微镜中的诱捕和主动移动。包括最近开发的动态多光束光学镊子在内的光学镊子在多个领域已被证明是必不可少的工具。我们演示了由四个激光束形成的光阱。在我们的差分主动光学操纵器（DAOM）中，光束沿四面体的轴排列，并在其交点附近大致准直。 DAOM充分利用了准直光的独特优势：与光学镊子相比，该仪器无需机械扫描即可使受限粒子在很长的距离内移动。光束的散射力叠加在它们的共同体积中，可以通过调整光束的强度来设置粒子上的总力（大小和方向）。可以针对每个光束快速且独立地完成此操作，从而使我们能够沿大约1nl的体积内的任意路径移动粒子。主动反馈确保可以根据需要将样品固定或运输到指定位置。这允许显微镜样品的聚焦和横向平移，这是显微镜中的常见任务。在操作期间，从四个侧面同时观察到粒子。这为3D显微镜结合观察中样品的非接触式操作开辟了新的视角。

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